Wedenberg C, Lindskog S
Scand J Dent Res. 1986 Aug;94(4):311-9. doi: 10.1111/j.1600-0722.1986.tb01769.x.
Attachment and spreading of phagocytes on a mineralized tissue surface is crucial for their proper resorptive function. In other studies, attachment and spreading have been shown to be highly dependent on the nature and composition of the surface. In the present study, peritoneal macrophages were cultured on infected and non-infected mineralized and non-mineralized dental tissues, which were examined with scanning electron microscopy at different observation periods. Although some cells had attached to non-infected predentin, only a few showed signs of spreading, even after long incubation times. This contrasted with the behavior of macrophages cultivated on enamel, mineralized dentin and infected predentin. Most of these cells showed spreading and the characteristics of active, phagocytosing cells. The reluctance of macrophages to spread on non-infected predentin was suggested to be due to the non-mineralized nature of this tissue, although an influence of endogenous resorption inhibitors cannot be excluded.
吞噬细胞在矿化组织表面的黏附和铺展对于其正常的吸收功能至关重要。在其他研究中，已表明黏附和铺展高度依赖于表面的性质和组成。在本研究中，将腹膜巨噬细胞培养在感染和未感染的矿化及非矿化牙组织上，并在不同观察期用扫描电子显微镜进行检查。尽管一些细胞已附着在未感染的前期牙本质上，但即使经过长时间孵育，也只有少数细胞显示出铺展迹象。这与在釉质、矿化牙本质和感染前期牙本质上培养的巨噬细胞的行为形成对比。这些细胞中的大多数显示出铺展以及活跃的吞噬细胞特征。巨噬细胞在未感染的前期牙本质上不愿铺展，这被认为是由于该组织的非矿化性质，尽管不能排除内源性吸收抑制剂的影响。
