新的欧洲鲶鱼完整线粒体基因组数据集及其特征
New complete mitogenome datasets and their characterization of the European catfish ().
作者信息
Székvári Kinga, Szabolcsi Zoltán, Kutasy Barbara, Hegedűs Géza, Virág Eszter
机构信息
Festetics Doctoral School, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.
Department of Plant Science and Biotechnology, Szent István University, i.e. the Predecessor of the Festetics Bioinnovation Group, Keszthely, Hungary.
出版信息
Data Brief. 2021 Sep 23;38:107418. doi: 10.1016/j.dib.2021.107418. eCollection 2021 Oct.
We present new complete mitogenome sequences of from 4 samples such as male and female individuals from two countries (Hungary, Czech Republic). The complete mitochondria were determined from genome sequencing by using Illumina MiSeq platform resulting in long, 300 bp. paired-end reads. De novo assembly was performed resulting in one nod (scaffold) covering the total mitochondria in each sample. The mitochondrial genomes were circular, double-stranded molecules of 16,524 bp in length and consisted of 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region. These sequences were deposited in the NCBI GeneBank under the accession numbers (MW796040, MW796041, MW796042, MW796043) and compared with the only available mitochondrial genome (NC_014261.1) sequenced by unidentified technology and showed 99% similarity. We found in seq1 82, in seq2 82, seq3 83, seq4 82 nucleotide alterations involving 10 protein-coding genes and meaning 29 amino acid substitutions as well.
我们展示了来自4个样本的新的完整线粒体基因组序列，这些样本来自两个国家（匈牙利、捷克共和国）的男性和女性个体。通过使用Illumina MiSeq平台进行基因组测序确定了完整的线粒体，产生了长度为300 bp的长配对末端读数。进行了从头组装，每个样本中产生了一个覆盖整个线粒体的节点（支架）。线粒体基因组是长度为16,524 bp的环状双链分子，由13个蛋白质编码基因（PCG）、2个核糖体RNA基因、22个转移RNA基因和1个控制区域组成。这些序列已存入NCBI基因库，登录号为（MW796040、MW796041、MW796042、MW796043），并与通过未知技术测序的唯一可用线粒体基因组（NC_014261.1）进行了比较，显示出99%的相似性。我们在序列1中发现了82个、序列2中发现了82个、序列3中发现了83个、序列4中发现了82个核苷酸改变，涉及10个蛋白质编码基因，也意味着29个氨基酸替换。
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