量化免疫染色的小鼠组织切片中分支结构的空间位置。
Quantifying spatial position in a branched structure in immunostained mouse tissue sections.
机构信息
Novo Nordisk Foundation Center for Stem Cell Biology (Danstem), University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
Institute of Translational Stem Cell Research, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
出版信息
STAR Protoc. 2021 Sep 28;2(4):100806. doi: 10.1016/j.xpro.2021.100806. eCollection 2021 Dec 17.
We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas. For complete details on the use and execution of this protocol, please refer to Nyeng et al. (2019).
我们开发了一种基于组织切片二维显微镜图像来定量细胞在分支结构中位置的方案。生物分支结构包括肺、肾和胰腺等器官。在这些器官中，基于定性估计，细胞命运与位置相关。然而，一直缺乏定量评估细胞位置的方法。通过该方案，我们在小鼠胚胎胰腺中测量了细胞命运与细胞位置之间的相关性。有关该方案使用和执行的完整详细信息，请参阅 Nyeng 等人。(2019)。
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