School of Allied Health, Faculty of Health Science, Curtin University, Perth, Western Australia, Australia.
School of Science, Engineering and Technology, RMIT University Vietnam, Ho Chi Minh City, Vietnam.
Microsc Res Tech. 2022 Feb;85(2):728-737. doi: 10.1002/jemt.23944. Epub 2021 Oct 10.
The nanostructural response of New Zealand white rabbit Achilles tendons to a fatigue damage model was assessed quantitatively and qualitatively using the endpoint of dose assessments of each tendon from our previous study. The change in mechanical properties was assessed concurrently with nanostructural change in the same non-viable intact tendon. Atomic force microscopy was used to study the elongation of D-periodicities, and the changes were compared both within the same fibril bundle and between fibril bundles. D-periodicities increased due to both increased strain and increasing numbers of fatigue cycles. Although no significant difference in D-periodicity lengthening was found between fibril bundles, the lengthening of D-periodicity correlated strongly with the overall tendon mechanical changes. The accurate quantification of fibril elongation in response to macroscopic applied strain assisted in assessing the complex structure-function relationship in Achilles tendons.
采用我们之前研究中对每条肌腱剂量评估的终点，对新西兰白兔跟腱的纳米结构响应进行定量和定性评估。在同一非存活完整肌腱中，同时评估力学性能变化和纳米结构变化。原子力显微镜用于研究 D 周期的伸长，并且在同一原纤维束内和原纤维束之间比较变化。由于应变增加和疲劳循环次数增加，D 周期增加。尽管在原纤维束之间没有发现 D 周期延长的显著差异，但 D 周期的延长与整个肌腱的机械变化密切相关。对宏观施加应变的原纤维伸长的准确量化有助于评估跟腱的复杂结构-功能关系。
