Douglas C M, Simpson J S, Holdaway I M
Aust N Z J Surg. 1986 Aug;56(8):645-9. doi: 10.1111/j.1445-2197.1986.tb04521.x.
Oestrogen receptors were measured by a histochemical technique in tumour tissue from 45 patients with breast cancer, and the results compared with receptor levels measured by a standard biochemical dextran-charcoal assay. Histochemical measurements were made by incubating frozen sections of tumour tissue with 17-fluoresceinated oestrone, using dithiothreitol to inhibit uptake by low affinity binding sites, and estimating receptor content by the extent of fluorescence seen microscopically. Results from the two assays gave varying estimates of receptor concentration, with 66% of patients classed as receptor-positive by the biochemical assay, and 44% of patients classed as receptor-positive using the histochemical technique. Because of these discrepancies the histochemical assay cannot be considered a direct substitute for biochemical receptor measurements. Progesterone receptor was detected by biochemical measurement in 80% of the subgroup of patients who were positive for oestrogen receptor in both assays. The histochemical assay may thus identify a subset of those patients considered oestrogen receptor-positive by conventional receptor assays, and this group may be more likely to respond to hormonal therapy. However, whether the histochemical method provides a predictive measurement for endocrine responsiveness which is superior to standard progesterone receptor assay remains to be determined.
采用组织化学技术对45例乳腺癌患者的肿瘤组织进行雌激素受体检测，并将结果与采用标准生化葡聚糖-活性炭分析法测定的受体水平进行比较。组织化学检测是通过将肿瘤组织的冰冻切片与17-荧光标记的雌酮孵育，使用二硫苏糖醇抑制低亲和力结合位点的摄取，并通过显微镜下观察到的荧光程度来估计受体含量。两种检测方法得出的受体浓度估计值不同，生化检测将66%的患者归类为受体阳性，而组织化学技术将44%的患者归类为受体阳性。由于这些差异，组织化学检测不能被视为生化受体检测的直接替代方法。在两种检测中雌激素受体均为阳性的患者亚组中，80%通过生化检测检测到孕激素受体。因此，组织化学检测可能会识别出那些通过传统受体检测被认为是雌激素受体阳性的患者中的一个子集，并且这个群体可能更有可能对激素治疗产生反应。然而，组织化学方法是否能提供优于标准孕激素受体检测的内分泌反应预测指标，仍有待确定。
