Guimarães Silva Vasconcelos Priscilla, Medeiros de Almeida Maia Carolina, Mendes de Vasconcelos Victor, Paolla Raimundo E Silva Joanda, Fechine Tavares Josean, Vieira Pereira Jozinete, Wanderley Cavalcanti Yuri, Maria Melo de Brito Costa Edja
Department of Dentistry, State University of Paraíba (UEPB), Campina Gande, Brazil.
Multi-user laboratory for Characterization and Analysis, Federal University of Paraíba (UFPB), João Pessoa, Brazil.
Gerodontology. 2022 Dec;39(4):366-373. doi: 10.1111/ger.12594. Epub 2021 Oct 11.
To evaluate the anti-fungal activity of Syzygium aromaticum essential oil and its inhibition of a multispecies biofilm in patients with oral candidiasis.
Inhibiting biofilm formation on the denture surface is an important practice for preventing denture stomatitis.
The anti-fungal activity against Candida albicans and non-albicans Candida species was evaluated through the microdilution method to define Minimal Inhibitory (MIC) and Fungicidal (MFC) Concentrations. Time-kill assay assessed growth kinetics of C. albicans based on pre-determined time points (0, 1, 2, 4, 6 and 24 hours). A multi-species biofilm was formed using human saliva from patients with oral candidiasis and anti-biofilm activity determined by Colony Forming Units per milliliter (CFU/mL) count, fluorescence microscopy with calcofluor white to observe yeast presence and structure, and metabolic activity by XTT (2,3-Bis-(2Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-carboxanilide) reduction assay.
The essential oil showed an anti-fungal activity against all Candida species (MIC 500-1000 µg/mL, MFC 1000-2000 µg/mL), and the time-kill assay showed that 2000 µg/mL (from 2 hours onward) and 1000 µg/mL (from 4 hours onward) concentrations had substantially lower yeast growth than the negative control. In the biofilm analysis, the essential oil had a lower CFU/mL count and a biofilm metabolic activity (91.4%) than seen with its negative control, and in both analyses, the essential oil was not significantly different from the positive control (chlorhexidine). Morphological analysis showed amorphous and fragmented cellular structures after treatment with the essential oil.
Syzygium aromaticum essential oil had anti-fungal activities, reduced the Candida growth kinetics substantially and inhibited the multi-species biofilm formation.
评估丁香精油的抗真菌活性及其对口腔念珠菌病患者多菌种生物膜的抑制作用。
抑制义齿表面生物膜形成是预防义齿性口炎的重要措施。
通过微量稀释法评估对白色念珠菌和非白色念珠菌的抗真菌活性，以确定最低抑菌浓度（MIC）和杀菌浓度（MFC）。时间杀菌试验根据预定时间点（0、1、2、4、6和24小时）评估白色念珠菌的生长动力学。使用口腔念珠菌病患者的人唾液形成多菌种生物膜，并通过每毫升菌落形成单位（CFU/mL）计数、用荧光增白剂进行荧光显微镜观察酵母的存在和结构以及通过XTT（2,3-双-(2-甲氧基-4-硝基-5-磺基苯基)-2H-四唑-5-甲酰苯胺）还原试验测定代谢活性来确定抗生物膜活性。
该精油对所有念珠菌属均显示出抗真菌活性（MIC为500 - 1000μg/mL，MFC为1000 - 2000μg/mL），时间杀菌试验表明，2000μg/mL（从2小时起）和1000μg/mL（从4小时起）浓度的酵母生长明显低于阴性对照。在生物膜分析中，该精油的CFU/mL计数和生物膜代谢活性（91.4%）均低于阴性对照，并且在两项分析中，该精油与阳性对照（氯己定）无显著差异。形态学分析显示，用该精油处理后细胞结构呈无定形且破碎。
丁香精油具有抗真菌活性，能显著降低念珠菌的生长动力学并抑制多菌种生物膜形成。
