Laboratory for Translational and Molecular Imaging, Cancer and Stem Cell Biology Programme, Duke-NUS Medical School; School of Materials Science and Engineering, Nanyang Technological University.
Singapore National Eye Centre; Singapore Eye Research Institute.
J Vis Exp. 2021 Sep 27(175). doi: 10.3791/62685.
Subconjunctival injection is an attractive route to administer ocular drugs due to easy trans-scleral access that bypasses anterior ocular barriers, such as the cornea and conjunctiva. While therapeutic effects and pharmacokinetics of the drugs upon subconjunctival injection have been described in some studies, very few assess the ocular distribution of drugs or drug delivery systems (DDS). The latter is critical for the optimization of intraocular DDS design and drug bioavailability to achieve the desired ocular localization and duration of action (e.g., acute versus. prolonged). This study establishes the use of fiberoptic confocal laser microendoscopy (CLM) to qualitatively study the ocular distribution of fluorescent liposomes in real-time in live mice after sub-conjunctival injection. Being designed for in vivo visual inspection of tissues at the microscopic level, this is also the first full description of the CLM imaging method to study spatio-temporal distribution of injectables in the eye after subconjunctival injection.
经结膜下注射是一种向眼部给药的有吸引力的途径，因为它可以绕过角膜和结膜等前眼部屏障，容易穿透巩膜。虽然一些研究已经描述了经结膜下注射后药物的治疗效果和药代动力学，但很少有研究评估药物或药物递送系统（DDS）的眼部分布。后者对于优化眼内 DDS 的设计和药物生物利用度以实现所需的眼部定位和作用持续时间（例如，急性与延长）至关重要。本研究建立了使用光纤共聚焦激光微内镜（CLM）实时定性研究活小鼠经结膜下注射后荧光脂质体的眼部分布。该方法专为在微观水平上对活体组织进行体内可视化检查而设计，也是首次全面描述 CLM 成像方法，以研究经结膜下注射后注射剂在眼部的时空分布。
