An NMR-based metabolomics study on sea anemones () with atrazine exposure.

Sea anemones have been recommended as critical bioindicators for marine environmental stressors; however, the understanding of the biological effects in response to sublethal pollutant exposure is still limited. In this study, NMR-based metabolomics was performed to investigate the effects of atrazine on with concentrations ranging from 3 to 90 ppb. As a result, the metabolic profiling of was significantly affected after 70 ppb treatment while a partial perturbation was observed as early as 3 ppb treatment. Glutamate was significantly changed at low atrazine concentrations with increased upregulation in concentrated atrazine experiments which is a potential biomarker for exposed to atrazine stressors. The TCA intermediates succinate and malate as well as the TCA cycle-related metabolites such as alanine, glycine, and taurine downregulated after atrazine treatment which also indicated the lower energy supply of In summary, our study demonstrated that significant metabolic level perturbation could be detected at low atrazine concentrations before a physical change could be observed, and glutamate or the nitrogen metabolism may be the initial target for sea anemones by atrazine. The study may provide pioneering results for using to predict the impacts of exposure to atrazine toxin in marine systems.