Institute of Medical Microbiology and Hygiene, University of Tuebingen, Tuebingen, Germany.
German Center for Infection Research (DZIF), partner site Tuebingen, Tuebingen, Germany.
Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005018.
Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequencing was performed followed by phylogenetic analysis based on the gene sequence and by whole genome datasets. The two strains compose a separate branch within the clade formed by the (ACB) complex with CIP 70.29 as the most closely related species. The average nucleotide identity compared to all other species of the ACB complex was below 94.2% and digital DNA-DNA hybridization values were less than 60%. Biochemical characteristics confirm affiliation to the ACB complex with some specific phenotypic differences. As a result of the described data, a new species is introduced, for which the name sp. nov. is proposed. The type strain is J00019 with a G+C DNA content of 38.8 mol% and it is deposited in the DSMZ Germany (DSM 111094) and CCUG Sweden (CCUG 74625).
从一对双胞胎的两个咽喉拭子中培养出两株非发酵、革兰氏阴性细菌分离株，这是在常规微生物监测筛查中进行的。由于这些分离株不能通过常规诊断方法明确鉴定，因此进行了全基因组测序，并基于 基因序列和全基因组数据集进行了系统发育分析。这两个菌株在由 （ACB）复合体形成的分支中构成一个单独的分支，与 CIP 70.29 关系最密切。与 ACB 复合体的所有其他物种相比，平均核苷酸同一性低于 94.2%，数字 DNA-DNA 杂交值低于 60%。生化特征证实与 ACB 复合体有关，具有一些特定的表型差异。由于描述的数据，引入了一个新的 物种，建议使用 sp. nov. 这个名称。其模式株为 J00019，G+C DNA 含量为 38.8 mol%，并保藏于德国 DSMZ（DSM 111094）和瑞典 CCUG（CCUG 74625）。
