Department of Bioengineering and Alcoholic Drink Technology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, Ménesi út 45, Hungary.
Department of Applied Biology, University of Science and Technology, Meghalaya 793101, India.
Bioresour Technol. 2022 Jan;343:126092. doi: 10.1016/j.biortech.2021.126092. Epub 2021 Oct 8.
In the downstream process, the bioconversion of lignocellulosic biomass can be improved by applying a biological pretreatment procedure using microorganisms to produce hydrolytic enzymes to modify the recalcitrant structure of lignocellulose. In this study, various Bacillus strains (B. subtilis B.01162 and B.01212, B. coagulans B.01123 and B.01139, B. cereus B.00076 and B.01718, B. licheniformis B.01223 and B.01231) were evaluated for the degrading capacity of wheat bran in the submerged medium using enzymatic activities, reducing sugars and weight loss as indicators. The obtained results revealed that the B. subtilis B.01162, B. coagulans B.01123 and B. cereus B.00076 could be promising degraders for the wheat bran pretreatment. Besides, the application of their consortium (the combination of 2-3 Bacillus species) showed the positive effects on cellulose bioconversion compared with monocultures. Among them, the mixture of B. subtilis B.01162 and B. coagulans B.01123 increased significantly the cellulase, endo-glucanase, and xylanase enzyme activity resulting in accelerating the lignocellulose degradation. Our results served a very good base for the development of microbial consortium for biological pretreatment of lignocellulosic raw materials.
在下游过程中，可以通过应用微生物生物预处理程序来提高木质纤维素生物质的生物转化效率，以产生水解酶来修饰木质纤维素的顽固结构。在这项研究中，评估了各种芽孢杆菌菌株（枯草芽孢杆菌 B.01162 和 B.01212、凝结芽孢杆菌 B.01123 和 B.01139、蜡样芽孢杆菌 B.00076 和 B.01718、地衣芽孢杆菌 B.01223 和 B.01231）对固态培养基中麦麸的降解能力，以酶活性、还原糖和失重率为指标。结果表明，枯草芽孢杆菌 B.01162、凝结芽孢杆菌 B.01123 和蜡样芽孢杆菌 B.00076 可能是麦麸预处理的有前途的降解菌。此外，它们的混合培养物（两种或三种芽孢杆菌的组合）的应用与单培养物相比显示出对纤维素生物转化的积极影响。其中，枯草芽孢杆菌 B.01162 和凝结芽孢杆菌 B.01123 的混合物显著提高了纤维素酶、内切葡聚糖酶和木聚糖酶的酶活性，从而加速了木质纤维素的降解。我们的研究结果为木质纤维素原料生物预处理微生物混合培养物的开发提供了很好的基础。
