Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on fluorescent probe.

Hydrogen peroxide (HO) is an important active oxygen species that plays a major role in redox balance and in physiological and pathological processes of various diseases of biological systems. As HO is an endogenous active molecule, fluctuations in HO content are not only affected by the state of biological system itself but also easily affected by Bisphenol A (BPA, a typical estrogenic environmental pollutant) in the external environment. Here, the near-infrared fluorescent probe Cy-NOH2 (λ = 750 nm) as a tool was synthesized to detect fluctuations in HO content in cells and organisms induced by BPA. High sensitivity and excellent selectivity were found when the probe Cy-NOH2 was used to monitor endogenous HO in vitro. In addition, the expression of HO induced by different concentrations of BPA was able to be detected by the probe. Zebrafish and mice models were induced with different concentrations of BPA, and the HO content showed significant increasing trends in zebrafish and livers of mice with increasing BPA concentrations. This study reveals that the probe Cy-NOH2 can be used as an effective tool to monitor the redox state in vivo under the influence of BPA, which provides a basis for clarifying the mechanisms of BPA in a variety of physiological and pathological processes.