一种用于在细胞和斑马鱼中追踪内源性和外源性 HO 的近红外荧光探针。
A near-infrared fluorescent probe for tracking endogenous and exogenous HO in cells and zebrafish.
机构信息
College of Science, China Agricultural University, Beijing 100193, PR China.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123158. doi: 10.1016/j.saa.2023.123158. Epub 2023 Jul 16.
HO is an important signaling molecule in the body, and its levels fluctuate in many pathological sites, therefore, it can be used as a biomarker for early diagnosis of disease. Since the environment in vivo is extremely complex, it is of great significance to develop a probe that can accurately monitor the fluctuation of HO level without interference from other physiological processes. Based on this, we designed and synthesized two new near-infrared HO fluorescent probes, LTA and LTQ, based on the ICT mechanism. Both of them have good responses to HO, but LTA has a faster response speed. In addition, the probe LTA has good biocompatibility, good water solubility, and a large Stokes shift (95 nm). The detection limit is 4.525 μM. The probe was successfully used to visually detect HO in living cells and zebrafish and was successfully used to monitor the changes in HO levels in zebrafish due to APAP-induced liver injury.
HO 是体内一种重要的信号分子，其水平在许多病理部位波动，因此可以作为疾病早期诊断的生物标志物。由于体内环境极其复杂，因此开发一种能够准确监测 HO 水平波动而不受其他生理过程干扰的探针具有重要意义。基于此，我们设计并合成了两种基于 ICT 机制的新型近红外 HO 荧光探针 LTA 和 LTQ。它们都对 HO 有很好的响应，但 LTA 的响应速度更快。此外，探针 LTA 具有良好的生物相容性、良好的水溶性和较大的斯托克斯位移（95nm）。检测限为 4.525μM。该探针成功用于活细胞和斑马鱼中 HO 的可视化检测，并成功用于监测由于 APAP 诱导的肝损伤导致斑马鱼中 HO 水平的变化。
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