Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n, 15782, Santiago de Compostela, Spain.
Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n, 15782, Santiago de Compostela, Spain; Facultad de Estudios Superiores Zaragoza, Universidad Nacional de México, Av. Guelatao 66, Ejército de Oriente, Iztapalapa, 09230, Ciudad de México, Mexico.
Talanta. 2022 Jan 1;236:122856. doi: 10.1016/j.talanta.2021.122856. Epub 2021 Sep 6.
In this study, a first attempt for isolating and determining (characterising) background levels of titanium dioxide nanoparticles (TiO NPs) in seaweed has been developed by using single particle inductively coupled plasma - mass spectrometry (SP-ICP-MS). Seaweeds were processed using an optimised ultrasound assisted extraction (UAE) procedure based on tetramethylammonium hydroxide (TMAH) before dilution and SP-ICP-MS analysis. The effect of the TMAH percentage in the extracting solution, as well as the volume of extracting solution and sonication (extraction) time, has been fully assessed. Additional experiments also showed that TiO NPs were quantitatively released from the seaweed matrix in one UAE step since the analysis of residues gave TiO NPs concentrations lower than the limit of quantification (LOQ) of the method. Validation of the method with 50 and 100 nm TiO NPs (10 μg L as Ti) showed good analytical recovery (115% and 112% for 50 and 100 nm TiO NPs, respectively), and good reproducibility (2% for size and 16% for number of TiO NPs). Experiments regarding TiO NPs stability showed that the extracted NPs are stable since there were not changes on the number of TiO NPs and TiO NPs size distributions when exposing TiO NPs standards to the optimised extractive conditions.
本研究首次尝试采用单颗粒电感耦合等离子体质谱法（SP-ICP-MS）从海藻中分离和测定（表征）二氧化钛纳米颗粒（TiO NPs）的本底水平。海藻经过优化的四甲基氢氧化铵（TMAH）超声辅助提取（UAE）程序处理，然后稀释并进行 SP-ICP-MS 分析。全面评估了 TMAH 在提取溶液中的百分比、提取溶液的体积和超声（提取）时间的影响。此外的实验还表明，TiO NPs 可以通过一次 UAE 步骤从海藻基质中定量释放，因为对残留物的分析给出的 TiO NPs 浓度低于方法的定量限（LOQ）。用 50nm 和 100nm TiO NPs（10μg L 作为 Ti）验证该方法，显示出良好的分析回收率（50nm 和 100nm TiO NPs 的回收率分别为 115%和 112%）和良好的重现性（粒径的重现性为 2%，TiO NPs 数量的重现性为 16%）。有关 TiO NPs 稳定性的实验表明，提取的 NPs 是稳定的，因为当将 TiO NPs 标准品暴露于优化的提取条件下时，TiO NPs 的数量和 TiO NPs 粒径分布没有变化。
