Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; State Key Laboratory of Estuarine and Coastal Research, Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, Ministry of Education & Shanghai, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, East China, Normal University, 500 Dongchuan Road, Shanghai 200241, China.
J Hazard Mater. 2022 Feb 15;424(Pt A):127383. doi: 10.1016/j.jhazmat.2021.127383. Epub 2021 Sep 30.
Quantitative characterization of nanoparticles (NPs) in marine shellfish is critical to understanding the risks of bio-accumulation. Based on single particle (sp)ICP-MS and electron microscopy, a standardized protocol was developed to extract Ag, Au, and indigenous Ti-containing NPs from mussels. The optimal parameters are: dry sample extraction with tetramethylammonium hydroxide (TMAH), 5% (v/v) final concentration of TMAH, extraction at 25 ℃ for 12 h, and separation by centrifugation (3000 rpm for 5 min). The particle number recoveries of spiked Ag and Au NPs were 88 ± 0.9% and 95 ± 1.1%, respectively, while Ti-containing NPs had a particle number concentration of 8.2 × 10 particles/mg and an average size of 70 nm in tested mussels. Furthermore, titanium oxide NPs, including rutile, anatase, and Magnéli phases (TiO) were found ubiquitously in 10 shellfish based on the optimal method. The particle number concentrations and average sizes of the Ti-containing NPs were 2.1 × 10-8.4 × 10 particles/mg and 70-80 nm, respectively. These Ti-containing NPs, such as TiO, accounted for about half of the Ti mass in shellfish, indicating that marine shellfish may be a significant sink for Ti-containing NPs.
定量描述海洋贝类中的纳米颗粒（NPs）对于了解生物积累的风险至关重要。基于单颗粒（sp）ICP-MS 和电子显微镜，开发了一种标准化方案从贻贝类中提取 Ag、Au 和本地产含 Ti 的 NPs。最佳参数为：使用四甲基氢氧化铵（TMAH）干燥样品提取，TMAH 的最终浓度为 5%（v/v），在 25℃下提取 12 小时，并通过离心（3000rpm 离心 5 分钟）分离。sp 掺杂的 Ag 和 Au NPs 的颗粒数回收率分别为 88±0.9%和 95±1.1%，而含 Ti 的 NPs 的颗粒数浓度为 8.2×10 个/mg，在测试的贻贝类中平均粒径为 70nm。此外，基于优化方法，在 10 种贝类中普遍发现了 TiO2 纳米颗粒，包括金红石、锐钛矿和 Magnéli 相（TiO）。含 Ti 的 NPs 的颗粒数浓度和平均粒径分别为 2.1×10-8.4×10 个/mg 和 70-80nm。这些含 Ti 的 NPs，如 TiO2，占贝类中 Ti 质量的一半左右，表明海洋贝类可能是含 Ti 的 NPs 的重要汇。
