Department of Urological Surgery, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
J Clin Lab Anal. 2021 Nov;35(11):e23973. doi: 10.1002/jcla.23973. Epub 2021 Oct 12.
NLRP3 inflammasome as a component of immune system has been found related to several cancers, but no study has assessed NLRP3 polymorphisms on risk of bladder cancer (BC). We aim to investigate whether NLRP3 polymorphisms are associated with the risk and clinical features of bladder cancer (BC) in a Chinese population.
Genotype frequency of two commonly studied NLRP3 SNPs (rs10754558 and rs35829419) was examined in 154 patients with BC and the 308 healthy controls. NLRP3 gene polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method.
The distribution frequencies of GG, AG+GG, GG, and G allele in NLRP3 (rs10754558) genotypes were significantly different between case and control group (OR = 2.296, P = .022; OR = 1.598, P = .020; OR = 1.998, P = .049; OR = 1.557, P = .006), but no statistical difference existed for rs35829419. Among smokers and alcohol drinkers, for rs10754558, individuals with AG, GG, and GG+AG genotypes had a higher BC risk compared with individuals with AA; for rs35829419, individuals with variant genotypes (AG and GG+AG) had a stronger risk of developing BC compared with individuals with AA (all P < .05). In stratified analyses of tumor size and tumor node metastasis, AG or GG genotypes of rs10754558 and rs35829419 SNPs were associated with BC risk (both P < .05).
NLRP3 polymorphisms (rs10754558 and rs35829419) were related to BC risk and tumor size and lymph node metastasis, especially among smokers and alcohol drinkers.
NLRP3 炎性小体作为免疫系统的一个组成部分，已被发现与多种癌症有关，但尚无研究评估 NLRP3 多态性与膀胱癌（BC）的风险。我们旨在研究 NLRP3 多态性是否与中国人群膀胱癌（BC）的风险和临床特征有关。
在 154 例 BC 患者和 308 例健康对照中，检测了两个常见 NLRP3 SNP（rs10754558 和 rs35829419）的基因型频率。NLRP3 基因多态性通过聚合酶链反应-限制性片段长度多态性方法进行基因分型。
NLRP3（rs10754558）基因型 GG、AG+GG、GG 和 G 等位基因的分布频率在病例组和对照组之间差异有统计学意义（OR=2.296，P=0.022；OR=1.598，P=0.020；OR=1.998，P=0.049；OR=1.557，P=0.006），但 rs35829419 无统计学差异。在吸烟者和饮酒者中，对于 rs10754558，与 AA 相比，AG、GG 和 GG+AG 基因型个体的 BC 风险更高；对于 rs35829419，与 AA 相比，变异基因型（AG 和 GG+AG）的个体患 BC 的风险更强（均 P<0.05）。在肿瘤大小和肿瘤淋巴结转移的分层分析中，rs10754558 和 rs35829419 SNP 的 AG 或 GG 基因型与 BC 风险相关（均 P<0.05）。
NLRP3 多态性（rs10754558 和 rs35829419）与 BC 风险以及肿瘤大小和淋巴结转移有关，尤其是在吸烟者和饮酒者中。
