不同辐照器对大鼠下颌骨放射性骨坏死模型建立的影响。
Effects of different irradiators on the establishment of osteoradionecrosis model of rat mandible.
机构信息
Dept. of Stomatology, Daping Hospital, Army Medical University, Chongqing 400042, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Oct 1;39(5):524-530. doi: 10.7518/hxkq.2021.05.005.
OBJECTIVES
To compare the effects of different irradiators on the establishment of osteoradionecrosis of jaw model (ORNJ) to explore an ideal modeling method.
METHODS
A total of 33 adult SD rats were included and randomly divided into three groups according to the radiation equipment, namely, the blank control (CN, 3 rats), group A (linear accelerator irradiation, 15 rats), and group B (small-animal irradiator irradiation, 15 rats). Groups A and B were irradiated with daily fractions of 7, 8, and 9 Gy for 5 days and further divided into three subgroups as follows: group A/B, 35 Gy; group A/B, 40 Gy; and group A/B, 45 Gy. The left mandibular molars of the rats were extracted 1 week after irradiation. The rats were sacrificed 3 weeks after tooth extraction, and the mandible specimens were obtained for gross observation, micro-CT scanning, and histological detection to evaluate the success rate of modeling.
RESULTS
At 3 weeks after dental extractions, complete gingival healing was found in the regions of dental extractions in groups A and A. However, failed gingival healing and bone exposure were found in groups A and B. Hematoxylin and eosin staining showed necrotic bone of the irradiated mandible in groups A, A,and B, with success modeling rates of 40% in group A and 93.3% in group B.
CONCLUSIONS
Small-animal irradiator irradiation is an ideal device for establishing ORNJ model.
目的
比较不同辐照器对建立颌骨放射性骨坏死模型（ORNJ）的影响，探索一种理想的建模方法。
方法
将 33 只成年 SD 大鼠纳入研究，并根据辐射设备随机分为三组，即空白对照组（CN，3 只大鼠）、A 组（直线加速器辐照，15 只大鼠）和 B 组（小动物辐照器辐照，15 只大鼠）。A 组和 B 组每天接受 7、8 和 9 Gy 的分次照射，连续照射 5 天，并进一步分为以下三个亚组：A/B 组，35 Gy；A/B 组，40 Gy；A/B 组，45 Gy。照射后 1 周，大鼠拔除左侧下颌磨牙。拔牙后 3 周处死大鼠，获取下颌骨标本进行大体观察、微 CT 扫描和组织学检测，以评估建模成功率。
结果
拔牙后 3 周，A 组和 A 组拔牙部位的牙龈均完全愈合。但 A 组和 B 组出现牙龈愈合不良和骨暴露。苏木精-伊红染色显示 A 组、A 组和 B 组照射下颌骨的坏死骨，A 组建模成功率为 40%，B 组为 93.3%。
结论
小动物辐照器辐照是建立 ORNJ 模型的理想设备。
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