鉴定并分子特征分析一株侵染玫瑰植株（月季）的新型卡罗病毒。
Identification and molecular characterization of a novel carlavirus infecting rose plants (Rosa chinensis Jacq.).
机构信息
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Department of Fruit Science, College of Horticulture, China Agricultural University, Beijing, 100193, China.
出版信息
Arch Virol. 2021 Dec;166(12):3499-3502. doi: 10.1007/s00705-021-05260-2. Epub 2021 Oct 12.
In the present study, the genome sequence of a potential novel virus, tentatively named "rose virus C" (RVC), was mined from publically available transcriptomic data from a Rosa chinensis plant. The complete genome sequence of RVC consists of 8,386 nt, excluding a 3' poly(A) tail, and contains five ORFs. Phylogenetic analysis showed that RVC clustered with members of the genus Carlavirus, family Betaflexiviridae. The replicase gene had 48.8-52.1% nt sequence identity to those of other carlaviruses, while the CP gene had 40.4-45.9% nt sequence identity, which is far below the species demarcation cutoff of 72%. The incidence of RVC in rose plants was low (5.4%). Overall, our data suggest that RVC is a novel atypical virus of the genus Carlavirus.
在本研究中，从 Rosa chinensis 植物的公开转录组数据中挖掘出一种潜在的新型病毒，暂命名为“玫瑰病毒 C”（RVC）。RVC 的完整基因组序列由 8386 个核苷酸组成，不包括 3' poly(A) 尾，包含五个 ORFs。系统发育分析表明，RVC 与卡尔病毒属的成员聚为一簇，属于贝塔病毒科。复制酶基因与其他卡尔病毒的核苷酸序列同一性为 48.8-52.1%，而 CP 基因的核苷酸序列同一性为 40.4-45.9%，远低于 72%的种间区分界限。RVC 在玫瑰植物中的发病率较低（5.4%）。总的来说，我们的数据表明 RVC 是卡尔病毒属的一种新型非典型病毒。
Zotero 插件