School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400700, China.
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Mikrochim Acta. 2021 Oct 12;188(11):376. doi: 10.1007/s00604-021-05043-1.
An AND-NAND logic pair is reported based on non-purified carbon quantum dots (CDs) for ascorbic acid (AA) detection. In the logic operation, molybdenum oxide nanosheets (MoO NSs) and AA are used as two signal inputs. In the presence of AA, MoO NSs are reduced to plasmonic molybdenum oxide, which decreases the CD fluorescence intensity because of a static quenching, dynamic quenching, and internal filtration effect. Meanwhile, the AA is oxidized to dehydroascorbic acid and forms fluorescent 3-(dihydroxyethyl) furo [3,4-b] quinoxaline-1-one with o-phenylenediamine from non-purified CDs. On this basis, an AND-NAND logic pair was constructed and used as a ratiometric fluorescence sensor for highly sensitive detection of AA. The method has a wide linear range of 0.05-50 μM, and a detection limit of 34 nM. In addition, it was used to detect AA in fresh fruit. Potential applications include chemical computing, optoelectronic devices, biomedical science, and environmental monitoring. HIGHLIGHTS: 1. A ratiometric fluorescence sensor based on AND-NAND logic pair constructed by CDs and MoO NSs was successfully fabricated. 2. The ratiometric fluorescence sensor exhibited satisfactory linear range, high sensitivity, and good selectivity for AA. 3. The ratiometric fluorescence method was able to detect AA in fresh fruit with good results comparable to official fluorescence methods.
一种基于未纯化碳量子点（CDs）的与非-与非逻辑对被报道用于检测抗坏血酸（AA）。在逻辑运算中，氧化钼纳米片（MoO NSs）和 AA 被用作两个信号输入。在 AA 存在的情况下，MoO NSs 被还原为等离子体氧化钼，由于静态猝灭、动态猝灭和内部过滤效应，降低了 CD 荧光强度。同时，AA 被氧化为脱氢抗坏血酸，并与未纯化 CDs 中的邻苯二胺形成荧光 3-（二羟乙基）呋喃[3,4-b]喹喔啉-1-酮。在此基础上，构建了与非-与非逻辑对，并将其用作比率荧光传感器，用于 AA 的高灵敏度检测。该方法具有 0.05-50 μM 的宽线性范围和 34 nM 的检测限。此外，它还被用于检测新鲜水果中的 AA。潜在的应用包括化学计算、光电设备、生物医学科学和环境监测。要点：1. 成功构建了基于 CDs 和 MoO NSs 的与非-与非逻辑对的比率荧光传感器。2. 该比率荧光传感器对 AA 表现出令人满意的线性范围、高灵敏度和良好的选择性。3. 比率荧光法能够检测新鲜水果中的 AA，结果与官方荧光法相当。
