Zhong Yaping, Zou Yibiao, Yang Xianhong, Lu Zhentan, Wang Dong
Key Laboratory of Textile Fiber and Products, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials & Application, Wuhan Textile University, Wuhan, 430200, China.
Mikrochim Acta. 2021 Dec 7;189(1):19. doi: 10.1007/s00604-021-05124-1.
A rapid and facile method is reported for the detection of ascorbic acid using molybdenum disulfide quantum dots (MoS QDs) as a fluorescence sensor. Water-soluble and biocompatible MoS QDs with the maximum fluorescence emission at 506 nm have been successfully synthesized by hydrothermal method and specific detection for ascorbic acid (AA) was constructed to utilize the modulation of metal ion on the fluorescence of MoS QDs and the affinity and specificity between the ligand and the metal ion. The fluorescence of MoS QDs was quenched by the irreversible static quenching of Fe through the formation of a MoS QDs/Fe complex, while the pre-existence of AA can retain the fluorescence of MoS QDs through the redox reaction between AA and Fe. Based on this principle, a good linear relationship was obtained in the AA concentration range 1 to 150 μM with a detection limit of 50 nM. The proposed fluorescent sensing strategy was proven to be highly selective, quite simple, and rapid with a requirement of only 5 min at room temperature (RT), which is particularly useful for rapid and easy analysis. Satisfactory results were obtained when applied to AA determination in fruits, beverages, and serum samples as well as AA imaging in living cells, suggesting its great potential in constructing other fluorescence detection and imaging platforms.
报道了一种快速简便的方法，该方法使用二硫化钼量子点（MoS QDs）作为荧光传感器来检测抗坏血酸。通过水热法成功合成了在506 nm处具有最大荧光发射的水溶性且生物相容性良好的MoS QDs，并利用金属离子对MoS QDs荧光的调制以及配体与金属离子之间的亲和力和特异性构建了对抗坏血酸（AA）的特异性检测方法。通过形成MoS QDs/Fe络合物，Fe的不可逆静态猝灭使MoS QDs的荧光猝灭，而预先存在的AA可通过AA与Fe之间的氧化还原反应保留MoS QDs的荧光。基于此原理，在1至150 μM的AA浓度范围内获得了良好的线性关系，检测限为50 nM。所提出的荧光传感策略被证明具有高度选择性、非常简单且快速，在室温（RT）下仅需5分钟，这对于快速简便的分析特别有用。将其应用于水果、饮料和血清样品中的AA测定以及活细胞中的AA成像时，获得了令人满意的结果，表明其在构建其他荧光检测和成像平台方面具有巨大潜力。
