Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.
Biogerontology. 2021 Dec;22(6):623-637. doi: 10.1007/s10522-021-09940-z. Epub 2021 Oct 12.
Cell surface glycoproteins, which are good indicators of cellular types and biological function; are suited for cell evaluation. Tissue remodeling using various cells is a key feature of regenerative therapy. For artificial heart remodeling, a mixture of heart constituent cells has been investigated for organ assembly, however, the cellular characteristics remain unclear. In this study, the glycan profiles of human cardiomyocytes (HCMs), human cardiac fibroblasts (HCFs), and human vascular endothelial cells (ECs) were analyzed using evanescent-field lectin microarray analysis, a tool of glycan profiling, to clarify the required cellular characteristics. We found that ECs had more "α1-2fucose" and "core α1-6fucose" residues than other cells, and that "α2-6sialic acid" residue was more abundant in ECs and HCMs than in HCFs. HCFs showed higher abundance of "β-galactose" and "β-N-acetylgalactosamine" residues on N-glycan and O-glycan, respectively, compared to other cells. Interestingly, cardiac glycan profiles were insignificantly changed with cellular senescence. The residues identified in this study may participate in organ maintenance by contributing to the preservation of glycan components. Therefore, future studies should investigate the roles of glycans in optimal tissue remodeling since identifying cellular characteristics is important for the development of regenerative therapies.
细胞表面糖蛋白是细胞类型和生物功能的良好指标，适合用于细胞评估。利用各种细胞进行组织重塑是再生治疗的一个关键特征。对于人工心脏重塑，已经研究了心脏成分细胞的混合物用于器官组装，但是细胞特性仍不清楚。在这项研究中，使用糖组学分析工具——瞬逝场凝集素微阵列分析，分析了人心肌细胞 (HCMs)、人心肌成纤维细胞 (HCFs) 和人血管内皮细胞 (ECs) 的聚糖图谱，以阐明所需的细胞特性。我们发现，ECs 比其他细胞具有更多的“α1-2 岩藻糖”和“核心 α1-6 岩藻糖”残基，而“α2-6 唾液酸”残基在 ECs 和 HCMs 中的丰度高于 HCFs。与其他细胞相比，HCFs 在 N-聚糖和 O-聚糖上分别具有更高的“β-半乳糖”和“β-N-乙酰半乳糖胺”残基丰度。有趣的是，心脏糖组图谱在细胞衰老过程中没有明显变化。本研究中鉴定的残基可能通过维持糖成分的完整性参与器官的维持。因此，未来的研究应该研究聚糖在最佳组织重塑中的作用，因为确定细胞特性对于再生治疗的发展很重要。
