Wallace H. Coulter Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, 1760 Haygood Dr. NE, HSRB Bldg., Suite E480, Atlanta, GA, 30322, USA.
Division of Pediatric Cardiology, Children's Healthcare of Atlanta Sibley Heart Center, Atlanta, GA, 30322, USA.
Curr Cardiol Rep. 2019 Aug 1;21(9):105. doi: 10.1007/s11886-019-1178-9.
Tissue engineering has expanded into a highly versatile manufacturing landscape that holds great promise for advancing cardiovascular regenerative medicine. In this review, we provide a summary of the current state-of-the-art bioengineering technologies used to create functional cardiac tissues for a variety of applications in vitro and in vivo.
Studies over the past few years have made a strong case that tissue engineering is one of the major driving forces behind the accelerating fields of patient-specific regenerative medicine, precision medicine, compound screening, and disease modeling. To date, a variety of approaches have been used to bioengineer functional cardiac constructs, including biomaterial-based, cell-based, and hybrid (using cells and biomaterials) approaches. While some major progress has been made using cellular approaches, with multiple ongoing clinical trials, cell-free cardiac tissue engineering approaches have also accomplished multiple breakthroughs, although drawbacks remain. This review summarizes the most promising methods that have been employed to generate cardiovascular tissue constructs for basic science or clinical applications. Further, we outline the strengths and challenges that are inherent to this field as a whole and for each highlighted technology.
组织工程已发展成为一个高度通用的制造领域，为推进心血管再生医学提供了巨大的前景。在这篇综述中，我们总结了目前用于体外和体内各种应用的功能性心脏组织的生物工程技术的最新进展。
过去几年的研究强烈表明，组织工程是推动患者特异性再生医学、精准医学、化合物筛选和疾病建模等加速领域的主要动力之一。迄今为止，已经使用了多种方法来生物工程功能性心脏构建体，包括基于生物材料的、基于细胞的和混合（使用细胞和生物材料）的方法。虽然使用细胞方法已经取得了一些重大进展，并且有多个正在进行的临床试验，但无细胞心脏组织工程方法也取得了多个突破，尽管仍然存在一些缺点。本综述总结了用于生成心血管组织构建体的最有前途的方法，这些方法可用于基础科学或临床应用。此外，我们概述了整个领域以及每个突出技术所固有的优势和挑战。
