Dept of Applied Stem Cell Technologies, TechMed Centre, University of Twente, The Netherlands.
Biomater Sci. 2019 Aug 20;7(9):3566-3580. doi: 10.1039/c8bm01348a.
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) generally have an immature fetal-like phenotype when directly compared to isolated CMs from human hearts, despite significant advance in differentiation of human pluripotent stem cells (hPSCs) to multiple cardiac lineages. Therefore, hPSC-CMs may not accurately mimic all facets of healthy and diseased human adult CMs. During embryonic development, the cardiac extracellular matrix (ECM) experiences a gradual assembly of matrix proteins that transits along the maturation of CMs. Mimicking these dynamic stages may contribute to hPSC-CMs maturation in vitro. Thus, in this review, we describe the progressive build-up of the cardiac ECM during embryonic development, the ECM of the adult human heart and the application of natural and synthetic biomaterials for cardiac tissue engineering with hPSC-CMs.
人多能干细胞衍生的心肌细胞（hPSC-CMs）与从人心脏中分离的心肌细胞直接相比，通常具有不成熟的胎儿样表型，尽管在将人多能干细胞（hPSCs）分化为多种心脏谱系方面取得了重大进展。因此，hPSC-CMs 可能无法准确模拟健康和患病的成年人类心肌细胞的所有方面。在胚胎发育过程中，心脏细胞外基质（ECM）经历了基质蛋白的逐渐组装，这些蛋白随着心肌的成熟而转变。模拟这些动态阶段可能有助于 hPSC-CMs 的体外成熟。因此，在这篇综述中，我们描述了胚胎发育过程中心脏 ECM 的逐步构建、成人心脏的 ECM 以及天然和合成生物材料在 hPSC-CMs 心脏组织工程中的应用。
