Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
Beijing Tong Ren Tang Health (Dalian) Seafoods Co., Ltd, Beijing, China.
Luminescence. 2022 Jan;37(1):82-88. doi: 10.1002/bio.4148. Epub 2021 Oct 28.
Nitrofurantoin (NFT), a typical highly effective nitrofuran antibiotic drug, has been prohibited but still widely found in animal food products. It can be metabolized in animals to form 1-amino-hydantoin (AHD) that can then form stable and toxic metabolite-protein adducts. Hence, the detection of NFT and AHD in aquatic products and feeds is very important. However, there are limited reports concerning NFT detection and none about AHD by using surface-enhanced Raman spectroscopy (SERS) method. Herein, potassium bromide (KBr) decorated silver (Ag) nanoparticles (Ag-BrNPs)-based SERS approach was proposed for NFT and AHD detection. The limit of detection (LOD) for NFT was 1 μg/L. The detection of NFT residues in sea cucumber and fish feeds was also realized with the LOD of 1 and 50 ng/g, respectively. More importantly, the sensing of AHD was easily realized with the SERS approach for the first time. After the derivatization with 2-nitrobenzaldehyde (2-NBA), Ag-BrNPs were also successfully utilized for AHD detection in sea cucumber with the LOD of 5 ng/g.
呋喃妥因（NFT）是一种典型的高效硝基呋喃类抗生素药物，已被禁用，但仍在动物食品中广泛发现。它在动物体内可代谢生成 1-氨基海因（AHD），然后形成稳定且有毒的代谢物-蛋白质加合物。因此，检测水产品和饲料中的 NFT 和 AHD 非常重要。然而，目前关于 NFT 的检测方法的报道很少，更没有使用表面增强拉曼光谱（SERS）方法检测 AHD 的报道。在此，我们提出了一种基于溴化钾（KBr）修饰的银（Ag）纳米粒子（Ag-BrNPs）的 SERS 方法来检测 NFT 和 AHD。NFT 的检测限（LOD）为 1μg/L。该方法还可实现海参和鱼饲料中 NFT 残留的检测，其 LOD 分别为 1 和 50ng/g。更重要的是，首次通过 SERS 方法实现了 AHD 的检测。经过 2-硝基苯甲醛（2-NBA）衍生化后，Ag-BrNPs 也可成功用于海参中 AHD 的检测，LOD 为 5ng/g。
