Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
College of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2021 Sep 23;22(19):10239. doi: 10.3390/ijms221910239.
Wheat powdery mildew, caused by the obligate parasite f. sp. , severely reduces wheat yields. Identifying durable and effective genes against wheat powdery mildew and further transferring them into wheat cultivars is important for finally controlling this disease in wheat production. has been widely used in wheat breeding programs in Southwest China due to the spectrum and potentially durable resistance to powdery mildew. In the present study, a resistance test demonstrated that is still effective against the race E20. We identified and cloned the with a total length of 4883 bp, including three exons and two introns, and encoded a protein carrying the CC-NBS-NBS-LRR domain in the -linked region flanked by two EST markers, and , by integrating analysis of gene annotation in wheat reference genome and both sequence and expression difference in available transcriptome data. Two missense mutations were detected at positions 68 and 83 in the CC domain. The results of both cosegregation linkage analysis and qRT-PCR also suggested that was a potential candidate gene of . This study allowed us to move toward the final successfully clone and apply in wheat resistance improvement by gene engineering.
小麦白粉病由专性寄生物 f. sp. 引起，严重降低了小麦产量。鉴定对小麦白粉病具有持久和有效抗性的基因，并进一步将其转入小麦品种中，对于最终控制小麦生产中的这种病害非常重要。 由于对白粉病具有广谱和潜在的持久抗性，已被广泛应用于中国西南地区的小麦育种计划中。在本研究中，抗性测试表明 对 E20 菌系仍然有效。我们通过整合分析小麦参考基因组中的基因注释以及可用转录组数据中的序列和表达差异，鉴定并克隆了全长为 4883bp 的 ，其包含三个外显子和两个内含子，并在 -连锁区域编码了一个带有 CC-NBS-NBS-LRR 结构域的蛋白质，该区域两侧有两个 EST 标记 和 。在 CC 结构域的 68 和 83 位检测到两个错义突变。共分离连锁分析和 qRT-PCR 的结果也表明 是 的一个潜在候选基因。本研究使我们能够朝着最终成功克隆并通过基因工程应用 来提高小麦抗性的方向前进。
