Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping.

Wheat powdery mildew, caused by the obligate parasite f. sp. , severely reduces wheat yields. Identifying durable and effective genes against wheat powdery mildew and further transferring them into wheat cultivars is important for finally controlling this disease in wheat production. has been widely used in wheat breeding programs in Southwest China due to the spectrum and potentially durable resistance to powdery mildew. In the present study, a resistance test demonstrated that is still effective against the race E20. We identified and cloned the with a total length of 4883 bp, including three exons and two introns, and encoded a protein carrying the CC-NBS-NBS-LRR domain in the -linked region flanked by two EST markers, and , by integrating analysis of gene annotation in wheat reference genome and both sequence and expression difference in available transcriptome data. Two missense mutations were detected at positions 68 and 83 in the CC domain. The results of both cosegregation linkage analysis and qRT-PCR also suggested that was a potential candidate gene of . This study allowed us to move toward the final successfully clone and apply in wheat resistance improvement by gene engineering.