Mocchetti I, Einstein R, Brosius J
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7221-5. doi: 10.1073/pnas.83.19.7221.
cDNA clones corresponding to the polypeptide that has been shown to be an endogenous diazepam binding inhibitor and may act as a physiological ligand for the benzodiazepine/beta-carboline receptor have been isolated from bacteriophage lambda recombinant libraries from rat hypothalamus, total brain, and liver. The clones contain an open reading frame corresponding to 87 amino acids. A signal sequence is not present. In addition to high levels of mRNA in various brain regions, RNA blot analysis reveals an abundance of diazepam binding inhibitor mRNA in many peripheral organs (e.g., testes, kidney, liver, and heart) that are known to be rich in peripheral benzodiazepine recognition sites. The size of the mRNA in all tissue examined is approximately 0.7 kilobase. Southern blot analysis of genomic DNA suggests the presence of about six genes in the rat, some of which may be pseudogenes.
已从大鼠下丘脑、全脑和肝脏的噬菌体λ重组文库中分离出与一种多肽相对应的互补DNA（cDNA）克隆，该多肽已被证明是一种内源性地西泮结合抑制剂，可能作为苯二氮䓬/β-咔啉受体的生理配体。这些克隆含有一个对应于87个氨基酸的开放阅读框。不存在信号序列。除了在各个脑区有高水平的信使核糖核酸（mRNA）外，RNA印迹分析显示，在许多已知富含外周苯二氮䓬识别位点的外周器官（如睾丸、肾脏、肝脏和心脏）中也有大量的地西泮结合抑制剂mRNA。所有检测组织中的mRNA大小约为0.7千碱基。基因组DNA的Southern印迹分析表明，大鼠中存在约六个基因，其中一些可能是假基因。
