Fornwald J A, Kuncio G, Peng I, Ordahl C P
Nucleic Acids Res. 1982 Jul 10;10(13):3861-76. doi: 10.1093/nar/10.13.3861.
The nucleotide sequence of the chick a-actin gene reveals that the gene is comprised of 7 exons separated by six very short intervening sequences (IVS). The first IVS interrupts the 73 nucleotide 5' untranslated segment between nucleotides 61 and 62. The remaining IVS interrupt the translated region at codons 41/42, 150, 204, 267, and 327/328. The 272 nucleotide 3' untranslated segment is not interrupted by IVS. The amino acid sequence derived from the nucleotide sequence is identical to the published sequence for chick a-actin except for the presence of a met-cys dipeptide at the amino-terminus. The IVS positions in the chick a-actin gene are identical to those of the rat a-actin gene. While there is partial coincidence of the IVS in the a-actin genes with the vertebrate b-actin genes and 2 sea urchin actin genes, there is no coincidence with actin genes from any other source except soybean where one IVS position is shared. This discordance in IVS positions makes the actin gene family unique among the eucaryotic genes analyzed to date.
鸡α-肌动蛋白基因的核苷酸序列显示，该基因由7个外显子组成，被6个非常短的间隔序列（IVS）隔开。第一个IVS在核苷酸61和62之间打断了73个核苷酸的5'非翻译区。其余的IVS在密码子41/42、150、204、267和327/328处打断翻译区。272个核苷酸的3'非翻译区未被IVS打断。从核苷酸序列推导的氨基酸序列与已发表的鸡α-肌动蛋白序列相同，只是在氨基末端存在一个甲硫氨酸-半胱氨酸二肽。鸡α-肌动蛋白基因中的IVS位置与大鼠α-肌动蛋白基因的相同。虽然α-肌动蛋白基因中的IVS与脊椎动物β-肌动蛋白基因和2个海胆肌动蛋白基因有部分重合，但除了大豆（有一个IVS位置相同）外，与其他任何来源的肌动蛋白基因都没有重合。IVS位置的这种不一致使得肌动蛋白基因家族在迄今为止分析的真核基因中独一无二。
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