Ricke Steven C, Feye Kristina M, Chaney W Evan, Shi Zhaohao, Pavlidis Hilary, Yang Yichao
Department of Food Science, Center of Food Safety, University of Arkansas, Fayetteville, AR, United States.
Diamond V, Cedar Rapids, IA, United States.
Front Microbiol. 2019 Jan 23;9:3280. doi: 10.3389/fmicb.2018.03280. eCollection 2018.
The accurate and rapid detection of spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific . Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques.
在美国，准确快速地检测[具体菌种名称]对于家禽加工全过程的优化监测至关重要。进一步开发高特异性和高灵敏度的检测方法以检测家禽基质中的[具体菌种名称]，对于减少食源性疾病具有巨大的实用价值和潜力。诸如聚合酶链反应（PCR）等分子方法的引入和发展，增强了食品行业在整个家禽生产过程中识别食源性病原体存在的诊断能力。各种方法的进一步创新，如基于免疫的分型和检测以及高通量分析，将提供重要的流行病学数据，如识别独特的或特定区域的[具体菌种名称]。与传统微生物学和富集技术相比，分子技术/方法有可能提高灵敏度和特异性，以及数据获取速度。本综述将重点关注用于识别和定量美国家禽中[具体菌种名称]的快速分子方法的开发和应用，以及比传统微生物技术更快、更精确的新方法的出现。
