Iwasaki K, Shiraga T, Tada K, Noda K, Noguchi H
Xenobiotica. 1986 Aug;16(8):717-23. doi: 10.3109/00498258609043562.
Age- and sex-related changes in sulphoconjugation were investigated in hepatic 105 000 g supernatants of three-week-old (young), seven-week-old (young adult), one-year-old (middle-aged) and two-year-old (old) Sprague-Dawley rats in the presence of biologically active sulphate. The supernatants of seven-week-old, one-year-old and two-year-old female rats catalysed the sulphoconjugations of alcohol (tiaramide as substrate), and alicyclic amine, alkylamine and arylamine more rapidly than those of the respective males, but the supernatants of three-week-old rats did not exhibit significant sex-related differences. The sulphoconjugations by the supernatants of female rats were substantially constant during ageing. On the other hand, the supernatants of seven-week-old male rats catalysed phenol (beta-naphthol as substrate) sulphoconjugation more rapidly than those of seven-week-old females, but the supernatants of two-year-old rats did not exhibit this sex-related difference. These results indicate that the substrates for sulphoconjugation could be divided into at least two groups: an alcohol and amine type, and a phenol type.
在具有生物活性硫酸盐存在的情况下，研究了三周龄（幼年）、七周龄（青年）、一岁（中年）和两岁（老年）的斯普拉格 - 道利大鼠肝脏105000g上清液中硫酸结合作用的年龄和性别相关变化。七周龄、一岁和两岁雌性大鼠的上清液催化醇（以替拉米特为底物）、脂环胺、烷基胺和芳胺的硫酸结合作用比相应雄性大鼠的上清液更快，但三周龄大鼠的上清液未表现出明显的性别相关差异。雌性大鼠上清液的硫酸结合作用在衰老过程中基本保持恒定。另一方面，七周龄雄性大鼠的上清液催化苯酚（以β-萘酚为底物）的硫酸结合作用比七周龄雌性大鼠的更快，但两岁大鼠的上清液未表现出这种性别相关差异。这些结果表明，硫酸结合作用的底物可至少分为两组：醇和胺类，以及酚类。
