The State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.
Autophagy. 2021 Dec;17(12):4499-4501. doi: 10.1080/15548627.2021.1972406. Epub 2021 Oct 13.
Formation of the double-membrane autophagosome requires membrane reorganization of the endomembrane system to generate membrane precursors. The ER-Golgi trafficking system has been shown to provide membranes for phagophore growth. Nonetheless, how the components of the ER-Golgi system are redirected toward autophagosome biogenesis remains unclear. Here, we identify a new type of membrane contact formed between the ER-Golgi intermediate compartment (ERGIC) and the ER-exit sites (ERES) under macroautophagy/autophagy-induction conditions. The ERGIC-ERES contact is established by the TMED9-PREB/SEC12 interaction and regulates the biogenesis of the ERGIC-COPII vesicles, which we found previously act as a membrane template for LC3 lipidation and autophagosome formation.
双膜自噬体的形成需要内膜系统的膜重排来产生膜前体。已经表明 ER-Golgi 运输系统为噬泡生长提供膜。然而，ER-Golgi 系统的成分如何被重定向到自噬体生物发生仍然不清楚。在这里，我们在巨自噬/自噬诱导条件下鉴定了 ER-Golgi 中间区（ERGIC）和 ER 出口部位（ERES）之间形成的一种新型膜接触。ERGIC-ERES 接触由 TMED9-PREB/SEC12 相互作用建立，并调节我们之前发现的 ERGIC-COPII 囊泡的生物发生，该囊泡作为 LC3 脂质化和自噬体形成的膜模板。
