Ge Liang, Schekman Randy
Department of Molecular and Cell Biology and Howard Hughes Medical Institute; University of California, Berkeley; Berkeley, CA USA.
Autophagy. 2014 Jan;10(1):170-2. doi: 10.4161/auto.26787. Epub 2013 Nov 11.
A long-standing quest in the autophagy field is to define the membrane origin of the autophagosome. We have established a cell-free assay based on LC3 lipidation that recapitulates multiple regulatory hallmarks of early autophagosome biogenesis. Using a systematic membrane fractionation approach, we have identified the ER-Golgi intermediate compartment (ERGIC) as the most efficient membrane substrate for LC3 lipidation. Further studies indicate that the ERGIC plays an essential role to trigger LC3 lipidation and autophagosome biogenesis by recruiting the key early autophagic factor ATG14.
自噬领域长期以来的一个探索目标是确定自噬体的膜来源。我们基于LC3脂化建立了一种无细胞检测方法，该方法概括了早期自噬体生物发生的多个调控特征。通过系统的膜分级分离方法，我们确定内质网-高尔基体中间区室（ERGIC）是LC3脂化最有效的膜底物。进一步的研究表明，ERGIC通过招募关键的早期自噬因子ATG14，在触发LC3脂化和自噬体生物发生中起关键作用。
