a The State Key Laboratory of Membrane Biology , Tsinghua University-Peking University Joint Center for Life Sciences , School of Life Sciences , Tsinghua University , Beijing , China.
Autophagy. 2018;14(5):918-920. doi: 10.1080/15548627.2018.1425053. Epub 2018 Apr 3.
Autophagosomal membrane sources generate autophagic membrane precursors, which later assemble into the double-membrane autophagosome. The key events happening on the membrane sources during autophagic membrane generation remain poorly characterized. Our previous work found the ER-Golgi intermediate compartment (ERGIC) as a membrane source for the phagophore, the precursor to the autophagosome. A relocation of the COPII machinery from the ER-exit sites (ERES) to the ERGIC generates vesicles for LC3 lipidation. In recent work, we made a further step by showing that a starvation-induced remodeling of ERES facilitates the relocation of COPII to the ERGIC and the generation of the autophagic membrane.
自噬体膜的来源产生自噬体膜前体，这些前体随后组装成双膜自噬体。自噬体膜生成过程中膜源上发生的关键事件仍未得到很好的描述。我们之前的工作发现内质网-高尔基体中间区（ERGIC）是噬泡的膜源，噬泡是自噬体的前体。COPII 机制从内质网出口位点（ERES）向 ERGIC 的重新定位产生了用于 LC3 脂质化的囊泡。在最近的工作中，我们更进一步地表明，饥饿诱导的 ERES 重塑促进了 COPII 向 ERGIC 的重新定位和自噬体膜的生成。
