Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.
Methods Mol Biol. 2022;2387:7-15. doi: 10.1007/978-1-0716-1779-3_2.
Immunohistochemistry (IHC) is a combination of immunological, biochemical, and pathological methods to visualize the presence and distribution of specific epitopes in tissue sections. Selected antigens are stained by differently labeled antibodies binding to their target antigens in situ. Here we describe sample preparation and sample staining in order to diagnose and analyze tissue samples infected with M. ulcerans from human as well as animal source.
免疫组织化学（IHC）是一种将免疫学、生物化学和病理学方法相结合的技术，用于在组织切片中可视化特定表位的存在和分布。选择的抗原通过与目标抗原在原位结合的不同标记抗体进行染色。在这里，我们描述了用于诊断和分析人类和动物来源的溃疡分枝杆菌感染组织样本的样品制备和样品染色方法。
