Biology and Genetics of Bacterial Cell Wall, Institut Pasteur, Paris, France.
INSERM, Equipe Avenir, Paris, France.
Methods Mol Biol. 2022;2387:117-130. doi: 10.1007/978-1-0716-1779-3_13.
Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.
Mycolactones 是由人类病原体溃疡分枝杆菌产生的聚酮合酶产物家族，最近被鉴定为新型宿主膜易位复合物（Sec61）抑制剂。本文提供了从细菌培养物中纯化 mycolactones 的方案，以及在生物样本中定量评估其的方法。
