测定溃疡分枝杆菌的生物能量学参数。

Determination of Bioenergetic Parameters in Mycobacterium ulcerans.

机构信息

NTU Institute for Health Technologies (HealthTech NTU), Interdisciplinary Graduate Programme, Nanyang Technological University, Singapore, Singapore.

Lee Kong Chian School of Medicine, Experimental Medicine Building, Nanyang Technological University, Singapore, Singapore.

出版信息

Methods Mol Biol. 2022;2387:219-230. doi: 10.1007/978-1-0716-1779-3_21.

DOI:10.1007/978-1-0716-1779-3_21

PMID:34643916

Abstract

The oxidative phosphorylation (OxPhos) pathway has emerged as an attractive pathway for the development of anti-mycobacterial drugs. The OxPhos pathway is essential for ATP resynthesis and maintenance of the electrochemical transmembrane gradient. The bioenergetic parameters of the pathway such as oxygen consumption rate and ATP levels are quantifiable using current technology. Measuring these parameters are useful tools to gauge rapidly the impact of drug candidates on their capacity to inhibit the OxPhos pathway in Mycobacterium ulcerans.

摘要

氧化磷酸化（OxPhos）途径已成为开发抗分枝杆菌药物的有吸引力的途径。OxPhos 途径对于 ATP 的重新合成和维持电化学跨膜梯度至关重要。该途径的生物能量学参数，如耗氧量和 ATP 水平，可使用当前技术进行量化。测量这些参数是快速评估候选药物抑制溃疡分枝杆菌 OxPhos 途径能力的有用工具。