Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang, 310058, People's Republic of China.
Department of Field Crop Pests, Plant Protection Research Institute, Agricultural Research Centre 12622, Dokki, Cairo, Egypt.
J Nanobiotechnology. 2021 Oct 13;19(1):318. doi: 10.1186/s12951-021-01068-z.
Despite developments in nanotechnology for use in the pharmaceutical field, there is still a need for implementation of this technology in agrochemistry. In this study, silver nanoparticles (AgNPs) were successfully prepared by a facile and an eco-friendly route using two different ligands, 2'-amino-1,1':4',1″-terphenyl-3,3″,5,5″-tetracarboxylic acid (HL) and 1,3,6,8-tetrakis (p-benzoic acid)-pyrene (TBAPy), as reducing agents. The physiochemical properties of the as-obtained AgNPs were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The toxicity of HL-AgNP and TBAPy-AgNP against the brown planthopper (BPH, Nilaparvata lugens) was also measured.
SEM and TEM analyses demonstrated the formation of quasi-spherical AgNP structures in the presence of HL and TBAPy. Insecticidal assays showed that TBAPy is less effective against N. lugens, with a median lethal concentration (LC) of 810 mg/L, while the toxicity of HL increased and their LC reached 786 mg/L 168 h posttreatment at a high concentration of 2000 mg/L. HL-AgNPs were also highly toxic at a low concentration of 20 mg/L, with LC = ~ 3.9 mg/L 168 h posttreatment, while TBAPy-AgNPs exhibited less toxicity at the same concentration, with LC = ~ 4.6 mg/L.
These results suggest that the synthesized AgNPs using the two ligands may be a safe and cheaper method compared with chemical insecticides for protection of rice plants from pests and has potential as an effective insecticide in the N. lugens pest management program.
尽管纳米技术在制药领域的应用已有进展，但在农业化学领域仍需要实施这项技术。在这项研究中，成功地通过一种简便且环保的途径使用两种不同的配体，2'-氨基-1,1':4',1″-三联苯-3,3″,5,5″-四羧酸（HL）和 1,3,6,8-四（对苯甲酰基）-芘（TBAPy），制备了银纳米粒子（AgNPs）。用扫描电子显微镜（SEM）、能量色散 X 射线（EDX）、X 射线衍射（XRD）和透射电子显微镜（TEM）对所得到的 AgNPs 的物理化学性质进行了表征。还测量了 HL-AgNP 和 TBAPy-AgNP 对褐飞虱（BPH，Nilaparvata lugens）的毒性。
SEM 和 TEM 分析表明，在 HL 和 TBAPy 的存在下形成了准球形 AgNP 结构。杀虫试验表明，TBAPy 对 N. lugens 的效果较差，LC50 为 810mg/L，而 HL 的毒性增加，在高浓度 2000mg/L 时，其 LC 达到 168 小时后 786mg/L。HL-AgNPs 在低浓度 20mg/L 时也具有高毒性，LC50 为 168 小时后约 3.9mg/L，而 TBAPy-AgNPs 在相同浓度下毒性较小，LC50 为 4.6mg/L。
这些结果表明，与化学杀虫剂相比，使用这两种配体制备的 AgNPs 可能是一种安全且更经济的保护水稻植物免受害虫侵害的方法，并且在褐飞虱防治计划中具有作为有效杀虫剂的潜力。
