Jarvis A P, Grdina T A, Sullivan M F
In Vitro Cell Dev Biol. 1986 Oct;22(10):589-96. doi: 10.1007/BF02623518.
A new microencapsulation technology, developed for the encapsulation of living cells, has been demonstrated to be useful for the study of growth and differential gene expression using Friend erythroleukemic cells cultured at high cell densities. Using this technology, cultures of FL Clone 745 cells were encapsulated within semipermeable membranes composed of cross-linked alginic acid and poly-l-lysine. Cell growth studies measuring total cell number demonstrated an average generation time of 8.5 h in 5% (vol/vol) microcapsule cultures vs. 8.0 h in suspension cultures. Similar microcapsule cultures were serially propagated for more than 90 cell generations (13 sequential passages) with no significant change in this growth rate. In addition, final culture densities of greater than 1.0 X 10(8) cells/ml of intracapsular volume were attained using a 3% (vol/vol) microcapsule culture in conjunction with a standard refeeding schedule. Comparison of the level of dimethyl sulfoxide-induced hemoglobin production in suspension and microcapsule cultures demonstrated that the total amount of hemoglobin produced on a per cell basis was comparable in both systems. Due to the retention characteristics of the semipermeable membrane, the concentration of detergent-released hemoglobin, relative to other released protein, was approximately twofold higher in microcapsule cultures than in control suspension cultures.
一种为活细胞封装而开发的新型微囊化技术，已被证明可用于研究使用高密度培养的弗氏红白血病细胞的生长和差异基因表达。利用这项技术，将FL克隆745细胞培养物封装在由交联海藻酸和聚-L-赖氨酸组成的半透膜内。测量总细胞数的细胞生长研究表明，在5%（体积/体积）微囊培养物中，平均世代时间为8.5小时，而在悬浮培养物中为8.0小时。类似的微囊培养物连续传代90多个细胞世代（13次连续传代），生长速率没有显著变化。此外，使用3%（体积/体积）微囊培养物并结合标准的再喂养方案，囊内体积的最终培养密度达到了大于1.0×10⁸个细胞/毫升。对悬浮培养和微囊培养中二甲基亚砜诱导的血红蛋白产生水平的比较表明，两个系统中每个细胞产生的血红蛋白总量相当。由于半透膜的保留特性，微囊培养物中去污剂释放的血红蛋白浓度相对于其他释放的蛋白质，比对照悬浮培养物中大约高两倍。
