Lima Leidiane Alencar de Oliveira, Miranda Giza Hellen Nonato, Aragão Walessa Alana Bragança, Bittencourt Leonardo Oliveira, Dos Santos Sávio Monteiro, de Souza Michel Platini Caldas, Nogueira Lygia S, de Oliveira Edivaldo Herculano Corrêa, Monteiro Marta Chagas, Dionizio Aline, Leite Aline Lima, Pessan Juliano Pelim, Buzalaf Marília Afonso Rabelo, Lima Rafael Rodrigues
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
Laboratory of Clinical Immunology and Oxidative Stress, Faculty of Pharmacy, Institute of Health Sciences, Federal University of Pará, Belém, Brazil.
Front Pharmacol. 2021 Sep 27;12:715394. doi: 10.3389/fphar.2021.715394. eCollection 2021.
Although fluoride (F) is well-known to prevent dental caries, changes in cell processes in different tissues have been associated with its excessive exposure. Thus, this study aimed to evaluate the effects of F exposure on biochemical, proteomic, and genotoxic parameters of submandibular glands. Twenty one old rats ( = 30) were allocated into three groups: 60 days administration of drinking water containing 10 mgF/L, 50 mgF/L, or only deionized water (control). The submandibular glands were collected for oxidative biochemistry, protein expression profile, and genotoxic potential analyses. The results showed that both F concentrations increased the levels of thiobarbituric acid-reactive substances (TBARS) and reduced glutathione (GSH) and changed the proteomic profile, mainly regarding the cytoskeleton and cellular activity. Only the exposure to 50 mgF/L induced significant changes in DNA integrity. These findings reinforce the importance of continuous monitoring of F concentration in drinking water and the need for strategies to minimize F intake from other sources to obtain maximum preventive/therapeutic effects and avoid potential adverse effects.
尽管氟化物（F）因预防龋齿而广为人知，但其过量暴露会导致不同组织中的细胞过程发生变化。因此，本研究旨在评估氟暴露对下颌下腺生化、蛋白质组学和遗传毒性参数的影响。将21只老年大鼠（n = 30）分为三组：分别饮用含10mgF/L、50mgF/L的水60天，或仅饮用去离子水（对照组）。收集下颌下腺进行氧化生物化学、蛋白质表达谱和遗传毒性潜力分析。结果表明，两种氟浓度均增加了硫代巴比妥酸反应性物质（TBARS）的水平，降低了谷胱甘肽（GSH）水平，并改变了蛋白质组学图谱，主要涉及细胞骨架和细胞活性。只有暴露于50mgF/L才会导致DNA完整性发生显著变化。这些发现强化了持续监测饮用水中氟浓度的重要性，以及需要采取策略尽量减少从其他来源摄入氟，以获得最大的预防/治疗效果并避免潜在的不良反应。
