Jackson R A, Lill P H, Gangemi J D
J Leukoc Biol. 1986 Nov;40(5):575-87. doi: 10.1002/jlb.40.5.575.
Intranasal inoculation of C3H/HeN mice with Propionibacterium acnes activates pulmonary macrophages but not splenic or peritoneal macrophages. When mice so treated were injected IV with tumor cells, no protection against the challenge was seen. Conversely, inoculation of C3H/HeN mice with P. acnes by the IP route activated splenic and peritoneal macrophages but not pulmonary macrophages. When mice with activated splenic and peritoneal macrophages were challenged with an IV injection of tumor cells, the mice demonstrated an increased resistance to the formation of pulmonary tumors. Thus, activation of splenic and peritoneal macrophages correlated better with protection against IV tumor challenge than activation of pulmonary macrophages. Experiments were done that demonstrated that the effect was not due to the augmentation of NK cell activity. The data are consistent with the conclusion that activated pulmonary macrophages alone are not effective in conferring resistance to pulmonary tumor nodule formation in this tumor model.
用痤疮丙酸杆菌经鼻内接种C3H/HeN小鼠可激活肺巨噬细胞，但不能激活脾巨噬细胞或腹腔巨噬细胞。当对如此处理的小鼠静脉注射肿瘤细胞时，未观察到对攻击的保护作用。相反，通过腹腔注射途径用痤疮丙酸杆菌接种C3H/HeN小鼠可激活脾巨噬细胞和腹腔巨噬细胞，但不能激活肺巨噬细胞。当用静脉注射肿瘤细胞攻击具有激活的脾巨噬细胞和腹腔巨噬细胞的小鼠时，这些小鼠对肺肿瘤形成的抵抗力增强。因此，与激活肺巨噬细胞相比，激活脾巨噬细胞和腹腔巨噬细胞与抵抗静脉注射肿瘤攻击的保护作用相关性更好。进行的实验表明，这种效应不是由于自然杀伤细胞活性的增强所致。这些数据与以下结论一致，即在该肿瘤模型中，单独激活的肺巨噬细胞在赋予对肺肿瘤结节形成的抗性方面无效。
