Doull J L, Vats S, Chaliciopoulos M, Stuttard C, Wong K, Vining L C
J Gen Microbiol. 1986 May;132(5):1327-38. doi: 10.1099/00221287-132-5-1327.
In Streptomyces venezuelae fertility, defined as chromosomal gene recombination, was enhanced over 1000-fold when one parent in a biparental conjugational cross lacked the physically-undetected plasmid SVP1, as compared with crosses in which both parents carried SVP1. The existence of SVP1 and at least two other fertility plasmids, SVP2 and SVP3, was detected in S. venezuelae by 'lethal zygosis' elicited by a plasmid-plus mycelium in contact with a plasmid-minus mycelium. Conjugational crosses were used to construct a linkage map of S. venezuelae which was highly consistent with the map of analogous loci in S. coelicolor A3(2). A cluster of genes governing chloramphenicol biosynthesis was located near arg, cys and pdxB genes at a position roughly equivalent to the 1-2 o'clock region of the S. coelicolor A3(2) map.
在委内瑞拉链霉菌中，当双亲接合杂交中的一方亲本缺乏物理上未检测到的质粒SVP1时，其定义为染色体基因重组的育性比双亲都携带SVP1的杂交提高了1000倍以上。通过质粒加菌丝体与无质粒菌丝体接触引发的“致死接合”，在委内瑞拉链霉菌中检测到了SVP1以及至少另外两种育性质粒SVP2和SVP3的存在。利用接合杂交构建了委内瑞拉链霉菌的连锁图谱，该图谱与天蓝色链霉菌A3(2)中类似位点的图谱高度一致。一组控制氯霉素生物合成的基因位于arg、cys和pdxB基因附近，位置大致相当于天蓝色链霉菌A3(2)图谱的1点至2点区域。
