D-核糖-L-半胱氨酸营养膳食补充剂对顺铂诱导的成年雄性Sprague-Dawley大鼠少弱畸精子症和生精上皮变性的改善潜力及生育能力增强作用。

Ameliorating potential and fertility enhancing activities of nutritional dietary supplementation of D-Ribose -l-Cysteine in cisplatin induced oligoasthenoteratozoospermia and seminiferous epithelium degeneration in adult male Sprague-Dawley rats.

作者信息

Adelakun Sunday Aderemi, Ogunlade Babatunde, Iteire Kingley Afoke, Adedotun Oluwafemi Abidemi

机构信息

Department Human Anatomy, Federal University of Technology, Akure, Nigeria.

Department of Anatomy, University of Medical Science, Ondo City, Nigeria.

出版信息

Metabol Open. 2021 Sep 28;12:100128. doi: 10.1016/j.metop.2021.100128. eCollection 2021 Dec.

DOI:10.1016/j.metop.2021.100128

PMID:34647003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8501771/

Abstract

BACKGROUND

Cisplatin (CN) is considered as a cytotoxic agent and DNA synthesis inhibitor. D-Ribose-l-Cysteine (DRLC) is an active ingredient of riboceine, help cells to produce glutathione on body demand.

AIM

Current study focused on ameliorating potential and fertility enhancing activities of D-Ribose-l-Cysteine in cisplatin induced oligoasthenoteratozoospermia and seminiferous epithelium degeneration.

MATERIALS AND METHOD

Sixty (60) male rats randomized into six groups of ten (n = 10) rats each. Group A (control) received 2.0 ml distilled water, group B received single dose of 8 mg/kg bwt CN, group C received 30 mg/kg bwt DRLC, group D received single dose of 8 mg/kg CN follow by 30 mg/kg bwt DRLC, group E received single dose of 8 mg/kg CN and vitamin C, group F received single dose of 8 mg/kg cisplatin follow by DRLC + Vit.C for 56 days. Parameters tested include: Sperm parameters, testosterone (TT), luteinizing hormone (LH), Follicle stimulating Hormone, (FSH), Prolactin, and testicular 17β-HSD activity, Blood hydroperoxide (BHP), Malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione (GSH) and Catalase (CAT), testicular histology and fertility test.

RESULTS

Cisplatin significantly reduced testicular volume, body weight, sperm quality, fertility indices, TT, FSH, LH, 17β-HSD, SOD, GSH, CAT, diameter and cross-sectional area of seminiferous tubules, spermatogenesis score. And elevate prolactin, testicular injury score, BHP and MDA compared with control group. Cisplatin only treated rats showed degenerated seminiferous epithelium with empty lumen. Intervention of D-Ribose-Cysteine ameliorated toxic impacts of CN on testis and improved the male fertility.

CONCLUSION

D-Ribose-l-Cysteine therefore, preserves testicular integrity and functions thereby, preventing the deleterious impact of CN.

摘要

背景

顺铂（CN）被认为是一种细胞毒性剂和DNA合成抑制剂。D-核糖-1-半胱氨酸（DRLC）是核糖霉素的一种活性成分，可帮助细胞根据身体需求产生谷胱甘肽。

目的

当前研究聚焦于D-核糖-1-半胱氨酸对顺铂诱导的少弱畸精子症和生精上皮变性的改善潜力及提高生育能力的活性。

材料与方法

将60只雄性大鼠随机分为6组，每组10只（n = 10）。A组（对照组）给予2.0 ml蒸馏水，B组给予单剂量8 mg/kg体重的顺铂，C组给予30 mg/kg体重的DRLC，D组先给予单剂量8 mg/kg体重的顺铂，随后给予30 mg/kg体重的DRLC，E组给予单剂量8 mg/kg体重的顺铂和维生素C，F组先给予单剂量8 mg/kg体重的顺铂，随后给予DRLC + 维生素C，持续56天。检测的参数包括：精子参数、睾酮（TT）、黄体生成素（LH）、卵泡刺激素（FSH）、催乳素、睾丸17β-羟类固醇脱氢酶活性、血液过氧化氢（BHP）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和过氧化氢酶（CAT）、睾丸组织学和生育力测试。

结果

与对照组相比，顺铂显著降低了睾丸体积、体重、精子质量、生育指数、TT、FSH、LH、17β-羟类固醇脱氢酶、SOD、GSH、CAT以及生精小管的直径和横截面积、精子发生评分。并提高了催乳素、睾丸损伤评分、BHP和MDA。仅接受顺铂治疗的大鼠显示生精上皮退化，管腔空虚。D-核糖-半胱氨酸的干预减轻了顺铂对睾丸的毒性影响并改善了雄性生育能力。