Department of Human Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria; Department of Anatomy, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Department of Human Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria.
Rev Int Androl. 2022 Oct;20 Suppl 1:S2-S16. doi: 10.1016/j.androl.2020.10.012. Epub 2022 Jan 31.
Available evidence suggests that cimetidine is a reproductive toxicant that induces sexual and testicular dysfunction. Ocimum gratissimum (OG) is globally consumed for medicinal and nutritional purposes. To determine the modulating role of aqueous leaf extract of Ocimum gratissimum on cimetidine-induced gonado-toxicity, sexually mature male rats were randomized into four groups of six (n=6) rats each. Group A: control given 2ml distilled water. Group B received 500mg/kg body weight (bwt) of OG extract, Group C received 50mg/kg bwt cimetidine, and group D received 50mg/kg bwt of cimetidine+500mg/kg bwt OG extract once daily for 8 weeks via gastric gavage. Parameters tested include sperm parameters, testosterone (TT), luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin, testicular alkaline phosphatase (ALP), acid phosphatase (ACP), lactate dehydrogenase (LDH), protein, cholesterol, glycogen, sexual behavioural parameters, and testicular histology.
There were depletions in the seminiferous epithelium, decreased sperm quality, TT, LH, and FSH, testicular enzymes, protein, cholesterol, glycogen, and sexual behaviour increase in animals treated with cimetidine only compared to control. OG restored and improved sexual behaviour and libido as evident from increased frequencies of mount, intromission, ejaculation, and ejaculatory latency. Mount latencies, intromission, post-ejaculation, and prolactin were significantly decreased. The significantly decreased testicular activities of ALP, ACP, LDH and protein, cholesterol, glycogen concentrations, TT, LH and FSH were increased by OG administration.
Ocimum gratissimum attenuated the deleterious effects of cimetidine on the testis, protected the seminiferous epithelium, restored, and boosted sexual competence, and promoted spermatogenesis.
现有证据表明，西咪替丁是一种生殖毒物，可引起生殖功能障碍和睾丸功能障碍。奥罗莫西坦（OG）在全球范围内被用于药用和营养目的。为了确定奥罗莫西坦的水提叶提取物对西咪替丁诱导的性腺毒性的调节作用，将性成熟雄性大鼠随机分为四组，每组 6 只（n=6）。A 组：给予 2ml 蒸馏水的对照组。B 组给予 500mg/kg 体重（bwt）OG 提取物，C 组给予 50mg/kg bwt 西咪替丁，D 组给予 50mg/kg bwt 西咪替丁+500mg/kg bwt OG 提取物，每日一次，通过胃灌胃给药，共 8 周。测试的参数包括精子参数、睾酮（TT）、促黄体生成素（LH）、促卵泡生成素（FSH）和催乳素、睾丸碱性磷酸酶（ALP）、酸性磷酸酶（ACP）、乳酸脱氢酶（LDH）、蛋白质、胆固醇、糖原、性行为参数和睾丸组织学。
与对照组相比，单独用西咪替丁处理的动物的生精上皮减少，精子质量下降，TT、LH 和 FSH、睾丸酶、蛋白质、胆固醇、糖原和性行为减少。OG 恢复并改善了性行为和性欲，表现为交配、插入、射精和射精潜伏期的频率增加。交配潜伏期、插入、射精后和催乳素显著降低。OG 给药可显著增加 ALP、ACP、LDH 和蛋白质、胆固醇、糖原浓度、TT、LH 和 FSH 的睾丸活性。
奥罗莫西坦减轻了西咪替丁对睾丸的有害影响，保护了生精上皮，恢复并提高了性能力，并促进了精子发生。
