一种用气相色谱-火焰离子化检测同时测定加热烟草制品中尼古丁、甘油、丙二醇和三醋酸甘油酯含量的简单方法。

A Simple Method to Simultaneously Determine the Level of Nicotine, Glycerol, Propylene Glycol, and Triacetin in Heated Tobacco Products by Gas Chromatography-Flame-Ionization Detection.

机构信息

Cigarette Testing Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078.

出版信息

J AOAC Int. 2022 Feb 4;105(1):46-53. doi: 10.1093/jaoacint/qsab140.

DOI:10.1093/jaoacint/qsab140

PMID:34648035

Abstract

BACKGROUND

Currently, there is no validated and available method internationally to determine the contents of nicotine and aerosolizing agents, namely glycerol and propylene glycol, added onto the heatsticks for use in heated smoking devices.

OBJECTIVE

To determine the concentrations of nicotine, propylene glycol, glycerol and triacetin in heated tobacco products (HTPs) which is essential to understanding their health effects on smokers as well as secondhand smokers.

METHODS

A simple methodology was developed and validated to simultaneously determine nicotine, propylene glycol, glycerol, and triacetin concentrations present in heatsticks. The tobacco material was extracted with a mixture of methanol-acetonitrile (7 + 3, by volume) with 1,3-butanediol and n-heptadecane as internal standards and analyzed with gas chromatography with flame-ionization detection (GC-FID).

RESULTS

Good linearity was achieved over the following concentration ranges: 0.1-1.0 mg/mL for nicotine, 0.03-2.0 mg/mL for propylene glycol, 0.5-10.0 mg/mL for glycerol, and 0.1-4.0 mg/mL triacetin, with a coefficient of determination ≥0.995. The limits of detection and quantification were 0.0009 and 0.003 mg/mL for nicotine, 0.02 and 0.02 mg/mL for propylene glycol, 0.03 and 0.09 mg/mL for glycerol, and 0.005 and 0.02 mg/mL for triacetin, respectively. Good recoveries were obtained for nicotine at 89.8-102.0%, propylene glycol at 95.5-102.5%, glycerol at 95.2-102.6%, and triacetin at 90.6-103.1%.

CONCLUSION

This method provides an affordable and reliable technique for routine analysis of nicotine and aerosolizing chemicals present in HTPs which is necessary to assess their impact to public health.

HIGHLIGHTS

Many gaps remain in research on HTPs, in particular, country levels information on the content of the products are limited. This article contains information on a newly developed method to simultaneously determine nicotine, propylene glycol, glycerol and triacetin present in the tobacco material and butts of heatsticks for HTPs.

摘要

背景

目前，国际上还没有经过验证和可用的方法来确定加热吸烟装置中使用的加热棒上添加的尼古丁和雾化剂（即甘油和丙二醇）的含量。

目的

确定加热烟草产品（HTP）中尼古丁、丙二醇、甘油和三醋酸甘油酯的浓度，这对于了解它们对吸烟者和二手烟者的健康影响至关重要。

方法

开发并验证了一种简单的方法，用于同时测定加热棒中存在的尼古丁、丙二醇、甘油和三醋酸甘油酯的浓度。用甲醇-乙腈（7+3，体积比）与 1,3-丁二醇和正十七烷的混合物提取烟草材料，并采用气相色谱-火焰离子化检测（GC-FID）进行分析。

结果

尼古丁在 0.1-1.0mg/mL、丙二醇在 0.03-2.0mg/mL、甘油在 0.5-10.0mg/mL 和三醋酸甘油酯在 0.1-4.0mg/mL 的浓度范围内获得了良好的线性关系，相关系数≥0.995。尼古丁的检测限和定量限分别为 0.0009 和 0.003mg/mL，丙二醇分别为 0.02 和 0.02mg/mL，甘油分别为 0.03 和 0.09mg/mL，三醋酸甘油酯分别为 0.005 和 0.02mg/mL。尼古丁的回收率为 89.8-102.0%，丙二醇为 95.5-102.5%，甘油为 95.2-102.6%，三醋酸甘油酯为 90.6-103.1%。