Ma Yaping, Xiao Qinlin, Li Li, Yang Juan, Wen Jing, Deng Yuyang, Guo Jia, Zhang Xixiang, Wen Wu, Shen Yi
Technology Center, China Tobacco Sichuan Industrial Co., Ltd., Chengdu, 610066, China.
Harmful Components and Tar Reduction in Tobacco Sichuan Key Laboratory, Chengdu, 610066, China.
Sci Rep. 2025 Jul 9;15(1):24603. doi: 10.1038/s41598-025-09092-x.
Moisture, 1,2-propanediol, and glycerol are crucial quality indicators in the production and application of novel tobacco products and reconstituted tobacco leaves. However, accurately measuring these components simultaneously remains a challenge. In this study, a method applying GC-TCD&FID method for the simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol was developed. The results showed that: (1) all four components exhibited good linearity, with correlation coefficients (R) above 0.995 for water and above 0.999 for three alcohols. Recoveries ranged from 94.49 to 102.83%, and repeatability RSD ranged from 1.25 to 2.33%. The detection limit for water was 0.32 mg/mL, and the alcohols ranged from 0.04 to 0.21 mg/mL. (2) Sample pretreatment and GC analysis were combined into a single step, reducing reagent use, workload, and total analysis time by about 50%. Anhydrous ethanol replaced methanol as the solvent, aligning the method with green and low-carbon principles. This approach has been validated and successfully applied in production over the past 3 years, demonstrating its reliability and practicality.
水分、1,2 - 丙二醇和甘油是新型烟草制品及再造烟叶生产与应用中的关键质量指标。然而，同时准确测定这些成分仍然是一项挑战。本研究开发了一种应用气相色谱 - 热导检测器和氢火焰离子化检测器（GC - TCD&FID）方法同时测定水分、1,2 - 丙二醇、甘油和薄荷醇的方法。结果表明：（1）所有四种成分均呈现良好的线性关系，水的相关系数（R）高于0.995，三种醇类的相关系数高于0.999。回收率在94.49%至102.83%之间，重复性相对标准偏差（RSD）在1.25%至2.33%之间。水的检测限为0.32 mg/mL，醇类的检测限在0.04至0.21 mg/mL之间。（2）将样品预处理和气相色谱分析合并为一个步骤，减少了试剂使用量、工作量，并将总分析时间缩短了约50%。无水乙醇替代甲醇作为溶剂，使该方法符合绿色低碳原则。该方法已在过去3年中得到验证并成功应用于生产，证明了其可靠性和实用性。
