Center for Clinical Pharmacology, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Division of Neonatology, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Biomed Chromatogr. 2022 Mar;36(3):e5262. doi: 10.1002/bmc.5262. Epub 2021 Nov 28.
Furosemide is a diuretic drug used to increase urine flow in order to reduce the amount of salt and water in the body. It is commonly utilized to treat preterm infants with chronic lung disease of prematurity. There is a need for a simple and reliable quantitation of furosemide in human urine. We have developed and validated an ultra-high performance liquid chromatography-tandem mass spectrometry method for furosemide quantitation in human urine with an assay range of 0.100-50.0 μg/ml. Sample preparation involved solid-phase extraction with 10 μl of urine. Intra-day accuracies and precisions for the quality control samples were 94.5-106 and 1.86-10.2%, respectively, while inter-day accuracies and precision were 99.2-102 and 3.38-7.41%, respectively. Recovery for furosemide had an average of 23.8%, with an average matrix effect of 101%. Furosemide was stable in human urine under the assay conditions. Stability for furosemide was shown at 1 week (room temperature, 4, -20 and -78°C), 6 months (-78°C), and through three freeze-thaw cycles. This robust assay demonstrates accurate and precise quantitation of furosemide in a small volume (10 μl) of human urine. It is currently being implemented in an ongoing pediatric clinical study.
呋塞米是一种利尿剂药物，用于增加尿液流量，以减少体内的盐和水。它通常用于治疗患有早产儿慢性肺疾病的早产儿。需要有一种简单可靠的方法来定量测定人尿中的呋塞米。我们已经开发并验证了一种超高效液相色谱-串联质谱法，用于定量测定人尿中的呋塞米，检测范围为 0.100-50.0μg/ml。样品制备涉及固相萃取，使用 10μl 尿液。质量控制样品的日内准确度和精密度分别为 94.5-106%和 1.86-10.2%，而日间准确度和精密度分别为 99.2-102%和 3.38-7.41%。呋塞米的平均回收率为 23.8%，平均基质效应为 101%。呋塞米在检测条件下在人尿中稳定。呋塞米在 1 周（室温、4°C、-20°C 和-78°C）、6 个月（-78°C）和三个冻融循环中表现出稳定性。这种强大的检测方法可准确、精密地定量测定 10μl 小体积人尿中的呋塞米。它目前正在一项正在进行的儿科临床研究中实施。
