College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277160, China.
College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277160, China.
Bioresour Technol. 2022 Jan;343:126113. doi: 10.1016/j.biortech.2021.126113. Epub 2021 Oct 11.
Soluble compounds produced during the enzymatic hydrolysis of lignocelluloses hampers cellulose conversion. Cellobiose and vanillin most severely inhibited the effect of cellobiohydrolase I. A concentration-dependent negative cooperative effect was found between cellobiose and vanillin. The combined inhibitory effect was about 83.5% of the cellobiose and 88.1% of the vanillin when their concentration was 20 mg/ml. However, the negative synergy could be eliminated by excessive enzyme loading. Differences in their binding sites on the catalytic domain of cellobiohydrolase I lead to negative synergistic inhibition, which should be considered in devising strategies to alleviate this effect. Combined β-glucosidase and PEG addition at an appropriate dose was feasible to balance cost and hydrolytic efficiency. To achieve efficient hydrolysis, especially at high solid concentrations, it is important to understand the synergistic inhibition between these inhibitors.
在木质纤维素的酶水解过程中产生的可溶性化合物会阻碍纤维素的转化。纤维二糖和香草醛对纤维素酶 I 的抑制作用最为严重。在浓度依赖性的情况下，发现纤维二糖和香草醛之间存在负协同效应。当它们的浓度为 20mg/ml 时，其组合抑制作用约为纤维二糖的 83.5%和香草醛的 88.1%。然而，通过过量的酶加载可以消除负协同效应。它们在纤维素酶 I 的催化结构域上的结合位点的差异导致了负协同抑制，在设计减轻这种效应的策略时应考虑到这一点。在适当的剂量下添加β-葡萄糖苷酶和 PEG 是可行的，可以平衡成本和水解效率。为了实现高效水解，特别是在高固体浓度下，了解这些抑制剂之间的协同抑制作用非常重要。
