Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country, UPV/EHU, P.O. Box 699, 48080 Bilbao, Spain.
Department of Stomatology II, Faculty of Medicine and Nursing, University of the Basque Country, UPV/EHU, P.O. Box 699, 48080 Bilbao, Spain.
Biomed Pharmacother. 2021 Nov;143:112218. doi: 10.1016/j.biopha.2021.112218. Epub 2021 Sep 28.
Oral candidiasis is frequently associated with Candida biofilms. Biofilms are microbial communities related to persistent, recalcitrant and difficult to-treat infections. Conventional treatments are not sufficient to overcome biofilm-associated candidiasis; thus, the search of new antifungal compounds is necessary. In the current study, we have evaluated the effect of three phytocompounds, carvacrol, cinnamaldehyde and thymol, against Candida planktonic and sessile cells. Reduction in biofilm biomass and metabolic activity was assessed during adhesion and mature biofilm phases. Candida albicans was the most biofilm-producing Candida species. All phytocompounds tested were fungicidal against Candida planktonic cells. Cinnamaldehyde was the most active in inhibiting biofilm adhesion, but carvacrol and thymol significantly reduced both mature biofilm biomass and metabolic activity. These results highlight the role of cinnamaldehyde, carvacrol and thymol as promising alternatives for the treatment of candidiasis due to their antibiofilm capacities, and stress the necessity to continue studies on their safety, toxicity and pharmacodynamics and pharmacokinetics.
口腔念珠菌病常与念珠菌生物膜有关。生物膜是与持续性、难治性和难以治疗的感染相关的微生物群落。传统治疗方法不足以克服生物膜相关的念珠菌病；因此，需要寻找新的抗真菌化合物。在目前的研究中，我们评估了三种植物化合物（香芹酚、肉桂醛和百里香酚）对浮游和定殖念珠菌细胞的影响。在黏附和成熟生物膜阶段评估了生物膜生物量和代谢活性的减少。白色念珠菌是产生物膜能力最强的念珠菌。所有测试的植物化合物对浮游念珠菌细胞均具有杀菌作用。肉桂醛在抑制生物膜黏附方面最有效，但香芹酚和百里香酚可显著降低成熟生物膜的生物量和代谢活性。这些结果强调了肉桂醛、香芹酚和百里香酚作为治疗念珠菌病的有前途的替代品的作用，因为它们具有抗生物膜能力，并强调需要继续研究它们的安全性、毒性以及药效学和药代动力学。
