Ziai R, Pan Y C, Hulmes J D, Sangameswaran L, Morgan J I
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8420-3. doi: 10.1073/pnas.83.21.8420.
By comparing the HPLC profiles of cerebellar extracts from adult and neonatal rats, a developmentally regulated polypeptide, termed PEP-19, was identified. The concentration of PEP-19 rose from 0.1 nmol/g of cerebellum at birth to 2 nmol/g at 20 days postpartum. The polypeptide could also be detected at lower levels in olfactory bulbs of adult rats but was absent in cerebral cortex, brain stem, and all non-neural tissues examined. HPLC-purified PEP-19 contained 61 amino acids and had a molecular size of 7.6 kDa. The native polypeptide is blocked at its amino terminus but was sequenced following proteolytic and chemical fragmentation. The primary amino acid sequence was determined to be: X (S-E) R Q S A G A T N G K D K T S G D N D G Q K K V Q E E F D I D M D A P E T E R A A V A I Q S Q F R K F Q K K K A G S Q S. PEP-19 has a unique sequence, but shares some homology with several calcium binding proteins including the beta chain of S100 and intestinal calcium binding protein. This polypeptide is the primary translation product of cerebellar poly(A)+ mRNA.
通过比较成年大鼠和新生大鼠小脑提取物的高效液相色谱（HPLC）图谱，鉴定出一种受发育调控的多肽，称为PEP - 19。PEP - 19的浓度从出生时小脑的0.1 nmol/g升至产后20天的2 nmol/g。该多肽在成年大鼠嗅球中也能以较低水平检测到，但在大脑皮层、脑干以及所有检测的非神经组织中均不存在。经HPLC纯化的PEP - 19含有61个氨基酸，分子大小为7.6 kDa。天然多肽的氨基末端被封闭，但在蛋白水解和化学裂解后进行了测序。确定其一级氨基酸序列为：X（S - E）R Q S A G A T N G K D K T S G D N D G Q K K V Q E E F D I D M D A P E T E R A A V A I Q S Q F R K F Q K K K A G S Q S。PEP - 19具有独特的序列，但与几种钙结合蛋白有一些同源性，包括S100的β链和肠钙结合蛋白。这种多肽是小脑多聚腺苷酸加尾（poly(A)+）mRNA的主要翻译产物。
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