禽肉瘤病毒转化基因产物磷酸化的一种细胞蛋白质底物的鉴定。

Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product.

作者信息

Erikson E, Erikson R L

出版信息

Cell. 1980 Oct;21(3):829-36. doi: 10.1016/0092-8674(80)90446-8.

Abstract

The avian sarcoma virus-transforming gene product (pp60src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60src. The phosphorylation of this protein (34K) in transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60src activity.

摘要

禽肉瘤病毒转化基因产物（pp60src）似乎有潜力通过磷酸化作用介导细胞转化，因为它与一种蛋白激酶活性紧密相关。我们进行了搜索并成功鉴定出一种正常细胞蛋白，它似乎是pp60src的底物。这种蛋白（34K）的磷酸化在禽源和哺乳动物源的ASV转化细胞中具有转化特异性。此外，34K多肽在体外可作为pp60src磷酸转移酶活性的底物，并在与体内主要磷酸化位点相同的位置被磷酸化。这些数据表明，在转化过程中，34000道尔顿的蛋白直接因pp60src的活性而被磷酸化。