Wu M, Allis C D, Richman R, Cook R G, Gorovsky M A
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8674-8. doi: 10.1073/pnas.83.22.8674.
An intervening sequence of 254 base pairs interrupts the coding region of the single gene for macronuclear histone H1 of the ciliated protozoan, Tetrahymena thermophila. The intervening sequence has splice junctions similar to those found in RNA polymerase II genes of other organisms. No obvious similarities are observed between this intron and the self-splicing intervening sequence of the Tetrahymena ribosomal gene. The derived amino acid sequence describes a small extremely basic H1 protein missing most of the central hydrophobic domain that is conserved in all other H1 proteins. Macronuclei divide amitotically, without chromosome condensation, suggesting the conserved globular domain of H1 plays a role in higher-order chromatin structure.
一段254个碱基对的间隔序列打断了纤毛原生动物嗜热栖热菌大核组蛋白H1单基因的编码区。该间隔序列具有与其他生物的RNA聚合酶II基因中发现的剪接连接相似的结构。在这个内含子与嗜热栖热菌核糖体基因的自我剪接间隔序列之间未观察到明显的相似性。推导的氨基酸序列描述了一种非常小的极端碱性H1蛋白，它缺失了在所有其他H1蛋白中保守的大部分中央疏水结构域。大核进行无丝分裂，没有染色体浓缩，这表明H1保守的球状结构域在高阶染色质结构中起作用。
