Takemura Masahiko, Lu Yi-Si, Nakato Eriko, Nakato Hiroshi
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis USA.
MicroPubl Biol. 2021 Aug 31;2021. doi: 10.17912/micropub.biology.000387. eCollection 2021.
Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in . In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the gene. Anti-HA antibody staining confirms that the distribution of the epitope-tagged Upd1::HA in various tissues is consistent with expression patterns revealed by previous studies. This transgenic fly strain will be useful in studying the expression, localization, and association partners of Upd1, and thus will contribute to understanding how activation of the JAK/STAT pathway is regulated.
Unpaired1（Upd1）是果蝇中Janus激酶/信号转导子及转录激活子（JAK/STAT）信号通路的一种配体。在本研究中，我们利用CRISPR/Cas9技术构建了一个转基因果蝇品系，其中血凝素（HA）表位标签序列被插入到Upd1基因的内源位点。抗HA抗体染色证实，表位标签化的Upd1::HA在各种组织中的分布与先前研究揭示的Upd1表达模式一致。该转基因果蝇品系将有助于研究Upd1的表达、定位及其相互作用蛋白，从而有助于理解JAK/STAT信号通路的激活是如何被调控的。
